Potential therapeutic effect of curcumin loaded hyalurosomes against inflammatory and oxidative processes involved in the pathogenesis of rheumatoid arthritis: The use of fibroblast-like synovial cells cultured in synovial fluid

• Published in: European journal of pharmaceutics and biopharmaceutics Vol. 136; pp. 84 - 92
• Main Authors: Manca, Maria Letizia, Lattuada, Donatella, Valenti, Donatella, Marelli, Ornella, Corradini, Costantino, Fernàndez-Busquets, Xavier, Zaru, Marco, Maccioni, Anna Maria, Fadda, Anna Maria, Manconi, Maria
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2019
• Subjects: Curcumin; IL-6 and IL-15; In vitro inflammation; Oxidative stress; Phospholipid vesicles; Synoviocytes; Phospholipid vesicles; Oxidative stress; Curcumin; IL-6 and IL-15; Synoviocytes; In vitro inflammation; synoviocytes; in vitro inflammation; phospholipid vesicles; oxidative stress; curcumin
• ISSN: 0939-6411; 1873-3441
• DOI: 10.1016/j.ejpb.2019.01.012

[Display omitted] In the present work curcumin loaded hyalurosomes were proposed as innovative systems for the treatment of rheumatoid arthritis. Vesicles were prepared using a one-step and environmentally friendly method. Aiming at finding the most suitable formulation in terms of size, surface charge and stability on storage, an extensive pre-formulation study was performed using different type and amount of phospholipids. Curcumin loaded vesicles prepared with 180 mg/ml of Phospholipon 90G (P90G) and immobilized with sodium hyaluronate (2 mg/ml) were selected because of their small size (189 nm), homogeneous dispersion (PI 0.24), negative charge (−35 mV), suitable ability to incorporate high amount of curcumin (E% ∼88%) and great stability on storage. The in vitro study using fibroblast-like synovial cells cultured in synovial fluid, demonstrated the ability of these vesicles to downregulate the production of anti-apoptotic proteins IAP1 and IAP2 and stimulate the production of IL-10, while the production of IL-6 and IL-15 and reactive oxygen species was reduced, confirming their suitability in counteracting pathogenesis of rheumatoid arthritis.