Development and validation of an HPLC-PDA method for the determination of myrsinoic acid B in the extracts of Rapanea ferruginea Mez

• Published in: Talanta (Oxford) Vol. 85; no. 2; pp. 1221 - 1224
• Main Authors: Baccarin, Thaisa, Muceneeki, Rodrigo S., Bresolin, Tania M.B., Yunes, Rosendo A., Malheiros, Ângela, Lucinda-Silva, Ruth M.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.08.2011; Elsevier
• Subjects: Alkenes - analysis; Analgesics - chemistry; Analgesics - standards; Analytical chemistry; Analytical validation; Arrays; Bark; Benzofurans - analysis; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, High Pressure Liquid - methods; Desiccation; Diodes; Drug Discovery; Drying; Exact sciences and technology; Flow rate; Liquid chromatography; Myrsinaceae - chemistry; Myrsinoic acid B; Other chromatographic methods; Phosphoric acid; Plant Bark - chemistry; Plant Extracts - chemistry; Plant Extracts - standards; Quality Control; Rapanea ferruginea; Standard deviation; Time Factors; Liquid chromatography; Rapanea ferruginea; Analytical validation; Myrsinoic acid B; Quality control; Trace analysis; Water; Phosphoric acid; HPLC chromatography; Biological marker; Method; Mobile phase; Rapaneaferruginea; Sample preparation; Detection limit; Acetonitrile; pH; Diode array; Standard deviation; Detection; Quantitative analysis; Methanol
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2011.05.017

New, simple, rapid and precise HPLC-PDA method has been developed and validated for quantification of biomarker myrsinoic acid B in stem bark extracts of Rapanea ferruginea Mez. The method employs a Phenomenex C18 column (250 mm × 4.6 mm I.D., 5 μm) with acetonitrile:methanol:water (pH 2.6 with phosphoric acid) at 48:30:22 as mobile phase, at a flow rate of 0.7 mL min −1 and photo diode array (PDA) detection at 270 nm. The validation data show that the method is specific, accurate, precise and robust. The method was linear, over a range of 5–100.0 μg mL −1, with a limit of detection of 0.369 μg mL −1 and limit of quantification of 1.233 μg mL −1. The method has also shown consistent recoveries (average of 101.3% and 0.12% RSD) of the biomarker, with low intra and inter-day relative standard deviation (1.26% and 1.62%, respectively). The evaluated hydroethanolic extract and dry extract presented MAB values of 63.53 and 36.07 mg g −1, respectively.