Melodinhenine B attenuates NLRP3 expression in a cerebral ischemia/reperfusion-induced neuronal injury rat model

• Published in: Folia neuropathologica Vol. 58; no. 1; pp. 30 - 37
• Main Authors: Li, Wei, Chen, Huisheng, Xu, Zhijia, Lv, Yan, Zhao, Ziai, Zhang, Jinghua, Qu, Fang
• Format: Journal Article
• Language: English
• Published: Poland Termedia sp. z o.o 01.01.2020; Termedia Publishing House
• Subjects: Animals; Anti-Inflammatory Agents - pharmacology; Blood-brain barrier; Brain - drug effects; Brain - pathology; Brain Ischemia - pathology; IL-1β; Indole Alkaloids - pharmacology; Inflammasomes; Inflammation; Ischemia; ischemia-reperfusion; Lymphocytes B; Male; melodinhenine b; Membrane permeability; neuronal injury; Neuroprotection; Neuroprotective Agents - pharmacology; NLR Family, Pyrin Domain-Containing 3 Protein - biosynthesis; NLR Family, Pyrin Domain-Containing 3 Protein - drug effects; Permeability; Rats; Rats, Sprague-Dawley; Recovery of Function - drug effects; Reperfusion; Reperfusion Injury - pathology; Rodents; Western blotting; Zonula occludens-1 protein; melodinhenine B; ischemia-reperfusion; inflammasomes; neuronal injury
• ISSN: 1641-4640; 1509-572X
• DOI: 10.5114/FN.2020.94004

This investigation evaluated the neuroprotective effect of melodinhenine B in a cerebral ischemia/reperfusion (I/R)-induced neuronal injury rat model. The effect of melodinhenine B was determined by evaluating the neurological deficit score, cerebral infarcted area, and blood-brain barrier (BBB) permeability. Moreover, the level of inflammatory cytokines and expression of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κβ), interleukin-1β (IL-1β), NLRP3, zonula occludens-1 (ZO-1), and occluding proteins were estimated by Western blotting. Histopathological changes and immunohistochemical analysis were performed to estimate the effect of melodinhenine B on neuronal injury. The neurological deficit score, percentage of infarcted area, and BBB permeability were improved in the melodinhenine B-treated group of rats. Treatment with melodinhenine B attenuated the altered expression of NF-κβ, IL-1β, NLRP3, ZO-1, and occluding proteins in the brain tissue of I/R-induced neuronal injury rats. The inflammatory cytokine levels were reduced in the melodinhenine B-treated group. Histopathologically, melodinhenine B reversed the pathological changes in the brain tissues of I/R-induced neuronal injury rats. In conclusion, melodinhenine B protects against neuronal injury in cerebral ischemia-reperfusion injury rats by regulating the inflammasomes.