Osteogenic Potential of Human Dental Pulp Stem Cells (hDPSCs) Growing on Poly L-Lactide-Co-Caprolactone and Hyaluronic Acid (HYAFF-11 TM ) Scaffolds

• Published in: International journal of molecular sciences Vol. 24; no. 23; p. 16747
• Main Authors: Bar, Julia K, Lis-Nawara, Anna, Kowalczyk, Tomasz, Grelewski, Piotr G, Stamnitz, Sandra, Gerber, Hanna, Klimczak, Aleksandra
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.12.2023
• Subjects: Biomedical materials; Bone marrow; Cell adhesion & migration; Cell Differentiation; Cell Proliferation; Cells, Cultured; Collagen; Dental Pulp; dental stem cells; Disease transmission; Growth factors; hDPSCs; Humans; HYAFF-11 scaffold; Hyaluronic acid; Hyaluronic Acid - pharmacology; Maxillofacial surgery; Mechanical properties; Minerals; Morbidity; Osteogenesis; PLCL scaffold; RNA, Messenger; Stem Cells; Tissue engineering; Tissue Scaffolds; PLCL scaffold; HYAFF-11 scaffold; osteogenesis; hDPSCs; dental stem cells
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms242316747

Bone tissue engineering using different scaffolds is a new therapeutic approach in regenerative medicine. This study explored the osteogenic potential of human dental pulp stem cells (hDPSCs) grown on a hydrolytically modified poly(L-lactide-co-caprolactone) (PLCL) electrospun scaffold and a non-woven hyaluronic acid (HYAFF-11™) mesh. The adhesion, immunophenotype, and osteogenic differentiation of hDPSCs seeded on PLCL and HYAFF-11™ scaffolds were analyzed. The results showed that PLCL and HYAFF-11™ scaffolds significantly supported hDPSCs adhesion; however, hDPSCs' adhesion rate was significantly higher on PLCL than on HYAFF-11™. SEM analysis confirmed good adhesion of hDPSCs on both scaffolds before and after osteogenesis. Alizarin red S staining showed mineral deposits on both scaffolds after hDPSCs osteogenesis. The mRNA levels of , , , and gene expression and their proteins were higher in hDPSCs after osteogenic differentiation on both scaffolds compared to undifferentiated hDPSCs on PLCL and HYAFF-11™. These results showed that PLCL scaffolds provide a better environment that supports hDPSCs attachment and osteogenic differentiation than HYAFF-11™. The high mRNA of early osteogenic gene expression and mineral deposits observed after hDPSCs osteogenesis on a PLCL mat indicated its better impact on hDPSCs' osteogenic potential than that of HYAFF-11™, and hDPSC/PLCL constructs might be considered in the future as an innovative approach to bone defect repair.