Enhancement of human mesenchymal stem cell infiltration into the electrospun poly(lactic-co-glycolic acid) scaffold by fluid shear stress

• Published in: Biochemical and biophysical research communications Vol. 463; no. 1-2; pp. 137 - 142
• Main Authors: Kim, Min Sung, Lee, Mi Hee, Kwon, Byeong-Ju, Koo, Min-Ah, Seon, Gyeung Mi, Park, Jong-Chul
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 17.07.2015
• Subjects: Biocompatible Materials - chemistry; Cell infiltration; Cell Movement; Cells, Cultured; Fluid shear stress; Human mesenchymal stem cell; Humans; Hydrodynamics; Lactic Acid - chemistry; Mechanotaxis; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - physiology; Microscopy, Electron, Scanning; PLGA scaffold; Polyglycolic Acid - chemistry; Tissue Engineering - instrumentation; Tissue Scaffolds - chemistry; Human mesenchymal stem cell; Cell infiltration; Fluid shear stress; PLGA scaffold; PLGA; hMSC; Mechanotaxis
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2015.05.048

The infiltration of the cells into the scaffolds is important phenomenon to give them good biocompatibility and even biodegradability. Fluid shear stress is one of the candidates for the infiltration of cells into scaffolds. Here we investigated the directional migration of human mesenchymal stem cells and infiltration into PLGA scaffold by fluid shear stress. The human mesenchymal stem cells showed directional migrations following the direction of the flow (8, 16 dyne/cm2). In the scaffold models, the fluid shear stress (8 dyne/cm2) enhanced the infiltration of cells but did not influence on the infiltration of Poly(lactic-co-glycolic acid) particles. •hMSCs migrated along the direction of the fluid shear stress.•hMSCs were infiltrated into PLGA scaffold by flow but PLGA micro-particles were not.•Mechanotaxis enhanced hMSC infiltration into PLGA scaffold.