Antioxidative constituents in Heterotheca inuloides

• Published in: Bioorganic & medicinal chemistry Vol. 5; no. 5; pp. 865 - 871
• Main Authors: Haraguchi, Hiroyuki, Ishikawa, Harumi, Sanchez, Yolanda, Ogura, Tetsuya, Kubo, Yumi, Kubo, Isao
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.05.1997
• Subjects: Animals; Antioxidants - isolation & purification; Antioxidants - pharmacology; Arnica - chemistry; Drug Evaluation, Preclinical; Flavonoids; Free Radical Scavengers - isolation & purification; Free Radical Scavengers - pharmacology; In Vitro Techniques; Kaempferols; Lipid Peroxidation - drug effects; Male; Microsomes, Liver - drug effects; Microsomes, Liver - metabolism; Mitochondria, Liver - drug effects; Mitochondria, Liver - metabolism; Naphthols - isolation & purification; Naphthols - pharmacology; Oxidative Stress - drug effects; Plants, Medicinal; Quercetin - analogs & derivatives; Quercetin - isolation & purification; Quercetin - pharmacology; Rats; Rats, Wistar; Sesquiterpenes - isolation & purification; Sesquiterpenes - pharmacology
• ISSN: 0968-0896; 1464-3391
• DOI: 10.1016/S0968-0896(97)00029-1

Sesquiterpenoids, 7-hydroxy-3,4-dihydrocadalin and 7-hydroxycadalin, and flavonoids, quercetin, kaempferol and their glycosides, isolated from Heterotheca inuloides (Asteraceae), a Mexican medicinal plant known as “arnica”, were evaluated as antioxidants. These compounds showed potent scavenging activity on diphenyl- p-picrylhydrazyl (DPPH) radical. Microsomal lipid peroxidation induced by Fe(III)-ADP/NADPH was inhibited by both terpenoids and flavonoids, though only flavonoids possessed superoxide anion scavenging activity in microsome. Flavonoids also scavenged enzymatically and non-enzymatically generated superoxide anion. On the other hand, mitochondrial lipid peroxidation induced by Fe(III)-ADP/NADH was inhibited only by sesquiterpenoids. Furthermore, these terpenes protected mitochondrial enzyme activity against oxidative stress. These results showed that two types of antioxidants existed in the dried flower of H. inuloides and would contribute to protection of tissues against various oxidative stresses. Sesquiterpenoids, 7-hydroxy-3,4-dihydrocadalin ( 1) and 7-hydroxycadalin ( 2), and flavonoids, quercetin, kaempferol and their glycosides, isolated from Heterotheca inuloides, a Mexican medicinal plant showed potent antioxidative activity against livermicrosomal and mitochondrial peroxidation. Especially, the terpene ( 1) protected mitochondrial enzyme activity against oxidative stress.