Antioxidant enzyme activity in the muscles of calves depleted of vitamin E or selenium or both

• Published in: British journal of nutrition Vol. 70; no. 2; pp. 621 - 630
• Main Authors: Walsh, Dominic M., Kennedy, D. Glenn, Goodall, Edward A., Kennedy, Seamus
• Format: Journal Article
• Language: English
• Published: Cambridge, UK Cambridge University Press 01.09.1993
• Subjects: Animals; Antioxidant enzymes; Biological and medical sciences; Catalase - metabolism; Cattle; Creatine Kinase - blood; Enzymatic Effects of Vitamin E or Selenium Depletion; Glucosephosphate Dehydrogenase - metabolism; Glutathione Reductase - metabolism; Glutathione Transferase - metabolism; Lipid peroxidation; Medical sciences; Metabolic diseases; Muscles - enzymology; Muscular Diseases - etiology; Other nutritional diseases (malnutrition, nutritional and vitamin deficiencies...); Selenium; Selenium - administration & dosage; Selenium - deficiency; Superoxide Dismutase - metabolism; Time Factors; Up-Regulation; Vitamin E; Vitamin E Deficiency - enzymology; Lipid peroxidation; Selenium; Vitamin E; Cattle; Antioxidant enzymes; Vitamin; Metabolic diseases; Ox; α-Tocopherol; Inorganic element; Congenital disease; Vertebrata; Striated muscle disease; Experimental disease; Mammalia; Oligoelement; Animal; Oxidation; Artiodactyla; Malnutrition; Ungulata; Nutritional deficiency; Nutritional status
• ISSN: 0007-1145; 1475-2662
• DOI: 10.1079/BJN19930153

Feeding diets depleted of vitamin E and Se to cattle can induce a disease known as nutritional degenerative myopathy. It is believed that an increased peroxidative challenge in muscle is involved in the pathogenesis of this disease. A number of species can up-regulate the activity of some antioxidant enzymes, including glutathione reductase (EC 1.6.4.2), glutathione transferase (EC 2.5.1.18), glucose-6-phosphate dehydrogenase (EC 1.1.1.49), catalase (EC 1.11.1.6), and superoxide dismutase (EC 1.15.1.1), in an attempt to mitigate the effects of a peroxidative challenge. A 2 × 2 factorial study was set up to examine possible changes in the activities of these antioxidant enzymes in muscles of ruminant calves fed on diets low in either vitamin E or Se. Four groups of four calves each were fed on a basal diet of NaOH-treated barley which was supplemented with α-tocopherol or Se or both for a total of 50 weeks. Calves fed on diets depleted of vitamin E, but not those ted on diets low in Se, developed subclinical myopathy, as judged by increases in the activity of plasma creatine kinase (EC 2.7.3.2), and had increased muscle concentrations of two indices of lipid peroxidation, namely thiobarbituric acidreactive substances, with and without ascorbate activation. Feeding diets depleted of vitamin E and diets low in Se both increased muscle activities of glucose-6-phosphate dehydregenase in heart, biceps and supraspinatus. This change may have occurred in an attempt to maintain intracellular pools of reduced glutathione. No other changes in antioxidant enzyme activity were observed.