Trichoplusia ni Transcriptomic Responses to the Phytosaponin Aglycone Hederagenin: Sex-Related Differences

• Published in: Journal of chemical ecology Vol. 50; no. 3-4; pp. 168 - 184
• Main Authors: Chen, Yinting, Lafleur, Christine, Smith, Ryan J., Kaur, Diljot, Driscoll, Brian T., Bede, Jacqueline C.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.04.2024; Springer Nature B.V
• Subjects: Agriculture; Alfalfa; Animals; Biochemistry; Biological Microscopy; Biomedical and Life Sciences; Butterflies & moths; Caterpillars; Cytochrome; Cytochrome P450; Cytochromes P450; Detoxification; Diet; Drug resistance; Ecology; Efflux; Entomology; Female; Females; Gender differences; Gene expression; Insecticides; Insects; Larva - drug effects; Larva - genetics; Legumes; Life Sciences; Male; Moths - drug effects; Moths - genetics; Moths - physiology; Nutrients; Oleanolic acid; Oleanolic Acid - analogs & derivatives; Oleanolic Acid - chemistry; Oleanolic Acid - metabolism; Oleanolic Acid - pharmacology; Oogenesis; Oxygenation; Pesticide resistance; Pesticides; Plant species; Protected species; Proteins; Saponins; Saponins - chemistry; Saponins - metabolism; Sex; Sex Characteristics; Sex differences; Transcriptome - drug effects; Transcriptomics; Trichoplusia ni; Trypsin; Cytochrome P; Transcriptomics; Toxicity; Antifeedant; Hederagenin; Saponin; Sex-specific; Cytochrome P450
• ISSN: 0098-0331; 1573-1561
• DOI: 10.1007/s10886-024-01482-1

Many plant species, particularly legumes, protect themselves with saponins. Previously, a correlation was observed between levels of oleanolic acid-derived saponins, such as hederagenin-derived compounds, in the legume Medicago truncatula and caterpillar deterrence. Using concentrations that reflect the foliar levels of hederagenin-type saponins, the sapogenin hederagenin was not toxic to 4th instar caterpillars of the cabbage looper Trichoplusia ni nor did it act as a feeding deterrent. Female caterpillars consumed more diet than males, presumably to obtain the additional nutrients required for oogenesis, and are, thus, exposed to higher hederagenin levels. When fed the hederagenin diet, male caterpillars expressed genes encoding trypsin-like proteins (LOC113500509, LOC113501951, LOC113501953, LOC113501966, LOC113501965, LOC113499659, LOC113501950, LOC113501948, LOC113501957, LOC113501962, LOC113497819, LOC113501946, LOC113503910) as well as stress-responsive (LOC113503484, LOC113505107) proteins and cytochrome P 450 6B2-like (LOC113493761) at higher levels than females. In comparison, female caterpillars expressed higher levels of cytochrome P 450 6B7-like ( LOC113492289 ). Bioinformatic tools predict that cytochrome P 450 s could catalyze the oxygenation of hederagenin which would increase the hydrophilicity of the compound. Expression of a Major Facilitator Subfamily (MFS) transporter ( LOC113492899 ) showed a hederagenin dose-dependent increase in gene expression suggesting that this transporter may be involved in sapogenin efflux. These sex-related differences in feeding and detoxification should be taken into consideration in insecticide evaluations to minimize pesticide resistance.