Gene trapping in Arabidopsis reveals genes involved in vascular development

The procambium is made up of stem cells that give rise to various vascular cells in plants. To understand the molecular nature of procambium cells, we tried to identify genes that characterize procambium cells using Arabidopsis gene trap lines. Among 26,000 gene trap lines, we found 67 lines in whic...

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Published inPlant and cell physiology Vol. 47; no. 10; pp. 1394 - 1405
Main Authors Nagawa, S.(Tokyo Univ. (Japan)), Sawa, S, Sato, S, Kato, T, Tabata, S, Fukuda, H
Format Journal Article
LanguageEnglish
Published Japan Oxford Publishing Limited (England) 01.10.2006
Subjects
Adenosine Triphosphatases - analysis
Adenosine Triphosphatases - genetics
Arabidopsis
Arabidopsis - genetics
Arabidopsis Proteins - analysis
Arabidopsis Proteins - genetics
ARABIDOPSIS THALIANA
Cotyledon - genetics
EXPRESION GENICA
EXPRESSION DES GENES
GENE
GENE EXPRESSION
Gene Expression Profiling
Gene Expression Regulation, Plant
GENES
Genome, Plant
Glucuronidase - analysis
Glucuronidase - genetics
IDENTIFICACION
IDENTIFICATION
Katanin
Meristem - genetics
Phloem - genetics
Plant Leaves - genetics
Staining and Labeling
TEJIDO VASCULAR
TISSU VASCULAIRE
VASCULAR TISSUES
Xylem - genetics
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Summary:The procambium is made up of stem cells that give rise to various vascular cells in plants. To understand the molecular nature of procambium cells, we tried to identify genes that characterize procambium cells using Arabidopsis gene trap lines. Among 26,000 gene trap lines, we found 67 lines in which beta-glucuronidase (GUS) staining occurred along vascular tissues in cotyledons and/or adult 1eaves. Although four gene trap lines showed procambium-preferential GUS expression, their expression patterns differed from each other during procambium development in root tips and young rosette leaves. Genomic regions flanking the gene trap insertion points in 25 of the 67 lines were determined, including three lines showing preferential GUS staining of the procambium. The three procambium-related genes encoded PINHEAD, katanin and an unknown DUF740 domain-containing protein. We discuss procambium development based on the functions and the differential GUS staining patterns of the procambium-related genes.
Bibliography:F60
F30
2007007897
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0032-0781
1471-9053
DOI:10.1093/pcp/pcl009