Adolescent alcohol binge drinking and withdrawal: behavioural, brain GFAP-positive astrocytes and acute methamphetamine effects in adult female rats

• Published in: Psychopharmacology Vol. 241; no. 8; pp. 1539 - 1554
• Main Authors: Costa, Priscila A., Everett, Nicholas A., Turner, Anita J., Umpierrez, Laísa S., Baracz, Sarah J., Cornish, Jennifer L.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.08.2024; Springer Nature B.V
• Subjects: Alcohol withdrawal; Alcoholic beverages; Animals; Anxiety; Anxiety - metabolism; Astrocytes; Astrocytes - drug effects; Astrocytes - metabolism; Behavior, Animal - drug effects; Binge Drinking - metabolism; Biomedical and Life Sciences; Biomedicine; Brain; Brain - drug effects; Brain - metabolism; Central Nervous System Stimulants - administration & dosage; Central Nervous System Stimulants - pharmacology; Drinking behavior; Ethanol; Ethanol - administration & dosage; Ethanol - pharmacology; Female; Females; Glial fibrillary acidic protein; Glial Fibrillary Acidic Protein - metabolism; Immunofluorescence; Locomotor activity; Methamphetamine; Methamphetamine - administration & dosage; Methamphetamine - pharmacology; Neurosciences; Original Investigation; Pharmacology/Toxicology; Psychiatry; Rats; Rats, Sprague-Dawley; Substance Withdrawal Syndrome - metabolism; Sucrose; Anxiety-like behaviour; Adolescence; Alcohol; Sucrose; Astrocytes; Methamphetamine
• ISSN: 0033-3158; 1432-2072; 1432-2072
• DOI: 10.1007/s00213-024-06580-2

Rationale Alcopop beverages are generally the first alcoholic beverage that young females drink which contain high levels of sugar and alcohol. The over-consumption of these drinks may encourage alcohol co-administration with methamphetamine (METH) impacting on drinking behaviour and glial function. Aims The aims of this study were to evaluate the effect of adolescent binge alcohol exposure on consumption level, anxiety-like behaviour, cross-sensitization with METH and on astrocyte expression in reward related brain regions. Methods Adolescent female Sprague-Dawley rats had daily 1-hour oral alcohol consumption of alcopop (ALCP; with sucrose) or ethanol-only (ETOH; without sucrose), transitioned from 5 to 15% (v/v) ethanol content for 34 days. Water and sucrose groups act as controls. During alcohol withdrawal, rats were tested for anxiety on the elevated plus maze (EPM) and locomotor activity following saline or METH (1 mg/kg i.p) treatment. Brains were then collected to assess astrocyte immunofluorescence for glial fibrillary acidic protein (GFAP) in reward-related brain regions. Results Rats pretreated with 5% ALCP consumed significantly more volume and ethanol intake when compared to 5% EtOH rats. Both ALCP and EtOH groups had a higher preference ratio for 5% than 15% alcohol solutions and ALCP rats had greater ethanol intake at 15% than EtOH rats. Alcohol withdrawal showed no significant differences between groups on anxiety, METH cross-sensitization effects or GFAP intensity in the regions studied. Conclusions Overall, the addition of sucrose to alcoholic solutions encouraged female rats to consume larger volumes and greater ethanol intake compared to ethanol-only solutions, yet did not have long lasting effects on behaviour and astrocytes.