Interleukin 1 Induces Human Marrow Stromal Cells in Long-Term Culture to Produce Granulocyte Colony-Stimulating Factor and Macrophage Colony-Stimulating Factor

• Published in: Blood Vol. 71; no. 2; pp. 430 - 435
• Main Authors: Fibbe, Willem E., Damme, Jo van, Billiau, Alfons, Goselink, Henrietta M., Voogt, Paul J., Eeden, Gerda van, Ralph, Peter, Altrock, Bruce W., Falkenburg, J.H. Frederik
• Format: Journal Article
• Language: English
• Published: Washington, DC Elsevier Inc 01.02.1988; The Americain Society of Hematology
• Subjects: Biological and medical sciences; Bone Marrow - metabolism; Bone Marrow Cells; Cell physiology; Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes; Cells, Cultured; Colony-Stimulating Factors - biosynthesis; Fundamental and applied biological sciences. Psychology; Granulocyte Colony-Stimulating Factor; Granulocyte-Macrophage Colony-Stimulating Factor; Growth Substances - biosynthesis; Humans; Immunologic Techniques; Interleukin-1 - pharmacology; Molecular and cellular biology; Time Factors; Human; Cell culture; Induction; Interleukin 1; Hematopoietic cell; Bone marrow; Biosynthesis; Stromal cell; Colony stimulating factor
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V71.2.430.430

Pure interleukin 1 (IL 1) was found to stimulate established human bone marrow stromal layers in long-term culture to produce colony-stimulating activity (CSA). Maximal concentrations in the culture medium were reached 24 hours after a single IL 1 pulse. The effect could be neutralized by a specific rabbit anti-IL 1 antiserum. Stromal layers, once stimulated by IL 1, continued to release CSA into the culture medium in the absence of exogenous IL 1, A second IL 1 pulse induced CSA release in an identical manner, as did the primary stimulation, indicating that the CSA released was actively produced. Using specific immunologic assays, both granulocyte colony-stimulating factor (G-CSF) and macrophage CSF (M-CSF) could be identified in the culture supernatants, and production of both factors was inducible by IL 1. Shortly after initiation of the long-term marrow cultures “spontaneous” G-CSF and M-CSF release occurred. The release of G-CSF diminished following addition of the anti-IL 1 antiserum, indicating that endogenous production of IL 1 by stromal cells had contributed to this effect. These results further support the role of IL 1 as an important modulator of CSF production by cells of the hematopoietic microenvironment.© 1988 by Grune &Stratton, Inc. 0006-4971/88/7102-002I$3.00/0