Intestinal microbial community diversity between healthy and orally infected rabbit with Entamoeba histolytica by ERIC-PCR

• Published in: Parasitology research (1987) Vol. 111; no. 3; pp. 1123 - 1126
• Main Authors: He, Guang-Zhi, Deng, Shu-Xuan, Qian, Ning
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.09.2012; Springer
• Subjects: Analysis; Animal, plant and microbial ecology; Animals; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; Cloning, Molecular; Diseases; Distribution; DNA - genetics; Entamoeba histolytica; Entamoebiasis - microbiology; Entamoebiasis - parasitology; Entamoebiasis - veterinary; Enterobacteriaceae - genetics; Erwinia amylovora; Escherichia coli; Fundamental and applied biological sciences. Psychology; General aspects; General aspects and techniques. Study of several systematic groups. Models; Health aspects; Immunology; Interspersed Repetitive Sequences - genetics; Intestines - microbiology; Invertebrates; Klebsiella pneumoniae; Mammalia; Medical Microbiology; Microbial ecology; Microbiology; Original Paper; Polymerase Chain Reaction - methods; Rabbits; Risk factors; Salmonella typhimurium; Shigella flexneri; Sinorhizobium meliloti; Various environments (extraatmospheric space, air, water); Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution; Xanthomonas campestris; Yersinia enterocolitica; Yersinia pestis; Yersinia Enterocolitica; Amebiasis; Entamoeba Histolytica; Basic Local Alignment Search Tool; Intestinal Microflora; Polymerase chain reaction; Protozoa; Vertebrata; Oryctolagus cuniculus; Lobosea; Mammalia; Rabbit; Parasite; Entamoeba histolytica; Lagomorpha; Microbial community
• ISSN: 0932-0113; 1432-1955
• DOI: 10.1007/s00436-012-2942-6

Enterobacterial repetitive intergenic consensus (ERIC)-PCR was applied to analyze the difference of intestinal microbial community diversity between healthy and orally infected rabbits with Entamoeba histolytica . The dynamic changes in different parts of digestive system including the duodenum, jejunum, ileum, caecum, and rectum in healthy and infected rabbits at different time points were also tested. The intestinal microbial community of the control healthy rabbits was steady, and the total number of ERIC-PCR bands in the control healthy rabbit was the least in the rectum and the most in the caecum. ERIC-PCR bands of orally inoculated rabbits did not obviously change until 24 h after postinoculation (p.i.). The numbers of the ERIC-PCR bands gradually decreased from 24 to 72 h p.i., and then, with the development of disease, the band numbers gradually increased until 6 days p.i. Sequence analysis showed that the nucleotide sequence homologies of the fragment about 1,200 bp of infected ileum sampled at 32 h p.i. were above 95 % with Sinorhizobium meliloti enterobacterial, Erwinia amylovora and Salmonella typhimurium , and the nucleotide sequence homologies of the fragment about 300 bp of infected ileum sampled at 48 h p.i. were more than 90 % with Xanthomonas campestris enterobacterial, Yersinia enterocolitica subsp., Shigella flexneri , S . meliloti enterobacterial, Yersinia pestis , Klebsiella pneumoniae subsp., and Escherichia coli . The prominent bacteria had changed after E . histolytica infection. The DNAstar of staple of ERIC-PCR showed that aerobe and facultative aerobe ( E . coli , Shigella , and Salmonella ) became preponderant bacilli in the intestine of orally infected rabbits with E . histolytica .