Pectin depolymerase [Pectic enzymes] activities associated with cell walls from Cicer arietinum L. epicotyl
The hydrolytic activity of the proteins extracted with 3 M LiCl from chick-pea (Cicer arietinum) cell walls to pectic fractions extracted with 50 mM trans-1,2-diaminocyclohexane-N,N,N',N'-tetraacetic acid (CDTA) and 50 mM sodium carbonate was studied. The pectic fractions contained acidic...
Saved in:
Published in | Plant and cell physiology Vol. 38; no. 11; pp. 1259 - 1263 |
---|---|
Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Oxford
Oxford University Press
1997
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | The hydrolytic activity of the proteins extracted with 3 M LiCl from chick-pea (Cicer arietinum) cell walls to pectic fractions extracted with 50 mM trans-1,2-diaminocyclohexane-N,N,N',N'-tetraacetic acid (CDTA) and 50 mM sodium carbonate was studied. The pectic fractions contained acidic polysaccharides with high molecular mass (higher than 5 x 10(3) kDa), mainly composed of uronic acids, galactose, arabinose and rhamnose. The extracted proteins depolymerized the pectic polysaccharides and also a commercial preparation of polygalacturonic acid from citrus, detected by a decrease in their viscosity and a shift of their molecular mass distribution. The extract was able to depolymerize a uronic acid-rich component in all the cases, although in different extent. Also, with regard to the CDTA-soluble pectins, a degradation of polyuronide and a shift of the molecular mass distribution of arabinogalactan was observed |
---|---|
AbstractList | The hydrolytic activity of the proteins extracted with 3 M LiCl from chick-pea (Cicer arietinum) cell walls to pectic fractions extracted with 50 mM trans-1,2-diaminocyclohexane-N,N,N',N'-tetraacetic acid (CDTA) and 50 mM sodium carbonate was studied. The pectic fractions contained acidic polysaccharides with high molecular mass (higher than 5 x 10(3) kDa), mainly composed of uronic acids, galactose, arabinose and rhamnose. The extracted proteins depolymerized the pectic polysaccharides and also a commercial preparation of polygalacturonic acid from citrus, detected by a decrease in their viscosity and a shift of their molecular mass distribution. The extract was able to depolymerize a uronic acid-rich component in all the cases, although in different extent. Also, with regard to the CDTA-soluble pectins, a degradation of polyuronide and a shift of the molecular mass distribution of arabinogalactan was observed The hydrolytic activity of the proteins extracted with 3 M LiCl from chick-pea (Cicer arietinum) cell walls to pec-tic fractions extracted with 50 mM trans-l,2-diaminocy-clohexane-N,N,N,N,-tetraacetic acid (CDTA) and 50 mM sodium carbonate was studied. The pectic fractions contained acidic polysaccharides with high molecular mass (higher than 5 × 103 kDa), mainly composed of uronic acids, galac-tose, arabinose and rhamnose. The extracted proteins depo-lymerized the pectic polysaccharides and also a commercial preparation of polygalacturonic acid from citrus, detected by a decrease in their viscosity and a shift of their molecular mass distribution. The extract was able to depolymerize a uronic acid-rich component in all the cases, although in different extent. Also, with regard to the CDTA-soluble pectins, a degradation of polyuronide and a shift of the molecular mass distribution of arabinogalactan was observed. The hydrolytic activity of the proteins extracted with 3 M LiCl from chick-pea (Cicer arietinum) cell walls to pectic fractions extracted with 50 mM trans-1,2-diaminocyclohexane-N,N,N,N-tetraacetic acid (CDTA) and 50 mM sodium carbonate was studied. The pectic fractions contained acidic polysaccharides with high molecular mass (higher than 5 x 10(3) kDa), mainly composed of uronic acids, galactose, arabinose and rhamnose. The extracted proteins depolymerized the pectic polysaccharides and also a commercial preparation of polygalacturonic acid from citrus, detected by a decrease in their viscosity and a shift of their molecular mass distribution. The extract was able to depolymerize a uronic acid-rich component in all the cases, although in different extent. Also, with regard to the CDTA-soluble pectins, a degradation of polyuronide and a shift of the molecular mass distribution of arabinogalactan was observed. |
Author | Zarra, I Acebes, J.L Sevillano, J.M. (Universidad de Leon (Spain)) |
Author_xml | – sequence: 1 fullname: Sevillano, J.M. (Universidad de Leon (Spain)) – sequence: 2 fullname: Zarra, I – sequence: 3 fullname: Acebes, J.L |
BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2147593$$DView record in Pascal Francis |
BookMark | eNpdkWtLHDEUhoNYcLX9CWKQfp1tbpNM_FaW1rUsKKggLSVkMidtdHYyJONl--sbXRHaT-f2nDfhPftod4gDIHRMyZwSzT_FJx9Tdxvv02D7PB_dOLeEaUr5DppRoWilSc130YwQziqiGrqH9nO-JaTknMzQ3QW4KQy4gzH2mzUkmwH_eGk6DMOf0so_sS3lQ5gCZGxzji7YCTr8GKbf2EHf40fb9xn7FNd4ERwkbFOAInu_xqs5hjG4OG369-idL7-ED6_xAF1__XK1WFar89OzxedV5QSTUyU80a1QyktgtQIvPRWukV3rVQvMC6kF5aK2UtKSCdV2ivBaCdfWzjom-QE62eq6FHNO4M2YwtqmjaHEPNtm_rXNFNvMq21l-eN2ebTZ2d4nO7iQ3xRYebDWz1i1xUKe4OltbNOdkYqr2ixvvpulPF2qG60NLfzRlvc2GvsrFcnrS0YoJ6xpqOasEIf_E98uqNZNOR2tKf8L6YaY9g |
CODEN | PCPHA5 |
CitedBy_id | crossref_primary_10_1039_C5RA13513C crossref_primary_10_3390_molecules15106948 crossref_primary_10_1007_s10854_017_7156_3 crossref_primary_10_3923_jps_2007_216_221 crossref_primary_10_1078_0176_1617_01153 |
ContentType | Journal Article |
Copyright | 1998 INIST-CNRS |
Copyright_xml | – notice: 1998 INIST-CNRS |
DBID | FBQ BSCLL IQODW AAYXX CITATION |
DOI | 10.1093/oxfordjournals.pcp.a029113 |
DatabaseName | AGRIS Istex Pascal-Francis CrossRef |
DatabaseTitle | CrossRef |
DatabaseTitleList | |
Database_xml | – sequence: 1 dbid: FBQ name: AGRIS url: http://www.fao.org/agris/Centre.asp?Menu_1ID=DB&Menu_2ID=DB1&Language=EN&Content=http://www.fao.org/agris/search?Language=EN sourceTypes: Publisher |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Biology Botany |
EISSN | 1471-9053 |
EndPage | 1263 |
ExternalDocumentID | 10_1093_oxfordjournals_pcp_a029113 2147593 ark_67375_HXZ_H6GH7X99_1 US201302881932 JP1998003151 |
GroupedDBID | --- -E4 -~X .2P .I3 0R~ 123 1TH 29O 2WC 4.4 482 48X 53G 5VS 5WD 6.Y 6P2 7.U 70D A8Z AABJS AABMN AAESY AAIMJ AAIYJ AAJKP AAJQQ AAKDD AAMDB AAMVS AANRK AAOGV AAPQZ AAPXW AAUQX AAVAP AAVLN AAWDT ABDBF ABEUO ABIXL ABJNI ABNKS ABPTD ABPTK ABQLI ABQTQ ABSAR ABSMQ ABWST ABXZS ABZBJ ACFRR ACGFS ACIWK ACKIV ACNCT ACPQN ACPRK ACUFI ACUTJ ADBBV ADEIU ADEYI ADEZT ADFTL ADGKP ADGZP ADHKW ADHZD ADIPN ADOCK ADORX ADQLU ADRIX ADRTK ADVEK ADYVW ADZTZ ADZXQ AEGPL AEJOX AEKPW AEKSI AELWJ AEMDU AENEX AENZO AEPUE AETBJ AEWNT AFFNX AFFZL AFGWE AFIYH AFOFC AFRAH AFXEN AFYAG AGINJ AGKEF AGKRT AGQXC AGSYK AHMBA AHXPO AIDBO AIJHB AIKOY AJEEA AKHUL AKWXX ALMA_UNASSIGNED_HOLDINGS ALUQC ALXQX ANFBD APIBT APJGH APWMN AQDSO ARIXL ASAOO ASPBG ATDFG ATTQO AVWKF AXUDD AYOIW AZFZN AZQFJ BAWUL BAYMD BCRHZ BEYMZ BHONS BQDIO BSWAC BYORX CAG CASEJ CDBKE COF CS3 CXTWN CZ4 DAKXR DFGAJ DIK DILTD DPORF DPPUQ DU5 D~K EBD EBS ECGQY EDH EE~ EJD ELUNK EMOBN ESTFP ESX F20 F5P F9B FBQ FEDTE FHSFR FLUFQ FOEOM FQBLK GAUVT GJXCC H5~ HAR HVGLF HW0 HZ~ IOX J21 KAQDR KBUDW KC5 KOP KQ8 KSI KSN M-Z M49 MBTAY N9A NGC NLBLG NOMLY NTWIH NU- NVLIB O0~ O9- OAWHX OBOKY ODMLO OHT OJQWA OJZSN OK1 OVD OWPYF O~Y P2P PAFKI PB- PEELM PQQKQ Q1. Q5Y QBD R44 RD5 RHF RNI ROL ROX ROZ RUSNO RW1 RXO RZF RZO SV3 TCN TEORI TLC TN5 TUS TWZ W8F WHG X7H Y6R YAYTL YKOAZ YNT YSK YXANX ZCG ZJWQK ZKX ~91 ~KM AARHZ AASNB ABMNT ABXVV ACMRT BSCLL JXSIZ 08R AAPBV H13 IQODW AAHBH AAUAY AAYXX ADQBN ATGXG CITATION |
ID | FETCH-LOGICAL-c426t-4f09b477f6e257ef6f14c86dbf7be2f46941345a66194147bd703574cb5cac263 |
ISSN | 0032-0781 |
IngestDate | Thu Sep 12 17:08:37 EDT 2024 Sun Oct 29 17:09:45 EDT 2023 Sun Mar 31 11:38:40 EDT 2024 Wed Dec 27 19:06:16 EST 2023 Wed Dec 27 19:14:16 EST 2023 |
IsDoiOpenAccess | false |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 11 |
Keywords | Enzyme Pectin Polygalacturonase Cell wall Hydrolysis Leguminosae Enzymatic activity Cicer arietinum Dicotyledones Glycosidases Angiospermae Hydrolases Isolation Spermatophyta O-Glycosidases Polysaccharide |
Language | English |
License | CC BY 4.0 |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c426t-4f09b477f6e257ef6f14c86dbf7be2f46941345a66194147bd703574cb5cac263 |
Notes | 1998003151 F60 istex:699AC0AF83B2DD13979A390B2808AA5EBA3E59D8 3 Author for correspondence. ArticleID:38.11.1259 ark:/67375/HXZ-H6GH7X99-1 |
OpenAccessLink | https://academic.oup.com/pcp/article-pdf/38/11/1259/5353131/38-11-1259.pdf |
PageCount | 5 |
ParticipantIDs | crossref_primary_10_1093_oxfordjournals_pcp_a029113 pascalfrancis_primary_2147593 istex_primary_ark_67375_HXZ_H6GH7X99_1 fao_agris_US201302881932 fao_agris_JP1998003151 |
PublicationCentury | 1900 |
PublicationDate | 1997-00-00 |
PublicationDateYYYYMMDD | 1997-01-01 |
PublicationDate_xml | – year: 1997 text: 1997-00-00 |
PublicationDecade | 1990 |
PublicationPlace | Oxford |
PublicationPlace_xml | – name: Oxford |
PublicationTitle | Plant and cell physiology |
PublicationYear | 1997 |
Publisher | Oxford University Press |
Publisher_xml | – name: Oxford University Press |
SSID | ssj0007830 |
Score | 1.5609703 |
Snippet | The hydrolytic activity of the proteins extracted with 3 M LiCl from chick-pea (Cicer arietinum) cell walls to pectic fractions extracted with 50 mM... The hydrolytic activity of the proteins extracted with 3 M LiCl from chick-pea (Cicer arietinum) cell walls to pec-tic fractions extracted with 50 mM... |
SourceID | crossref pascalfrancis istex fao |
SourceType | Aggregation Database Index Database Publisher |
StartPage | 1259 |
SubjectTerms | Arabinogalactan arabinose Biological and medical sciences Cell wall cell wall components CELL WALLS CICER ARIETINUM enzyme activity Enzymes epicotyls Fundamental and applied biological sciences. Psychology galactans galactose GLICOSIDASAS GLYCOSIDASE GLYCOSIDASES hydrolysis Metabolism PARED CELULAR PAROI CELLULAIRE Pectin Pectin-depolymerase PECTINAS PECTINE PECTINS Plant physiology and development polygalacturonase polymerization Polyuronide polyuronides protein composition rhamnose uronic acids |
Title | Pectin depolymerase [Pectic enzymes] activities associated with cell walls from Cicer arietinum L. epicotyl |
URI | https://api.istex.fr/ark:/67375/HXZ-H6GH7X99-1/fulltext.pdf |
Volume | 38 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9NAEF6lBSQuFRSqhlK0B8QlsnHs9WOPbgiEEpWqbaQoF8u78XIgxFHiAOHKH2dm17EdKOJxsSLbWScz387L8yDkucMzyWToW6GSjsXAh7DAV-GWCjLmSgkqN9NZvhfBYMTOx_641freyFpaF8KW326tK_kfrsI54CtWyf4DZ6tF4QR8Bv7CETgMx7_i8SVKK53Nms82GF1ameYYn3Wb1E5akr7KMMc43Zd0NluZqpIeCIllB51lWGX9qTO0O9kCkFFsZk2bFecamTx0vYCOhewE4zFeNRzGF-81JOw6wDqJr67indBs3Ouf9a_L-4Z1yAETUWrX9PYixqaA9TCZ1UxhsTMjU0H_WdwxPYG3QteLmuDqNkQoWFy8oY67rhGAv4h60wYr17-o5MnKXsiFnTouSHCvVnDbl_o_6b0qGxFHNfnc2yN33JD7OIDh1dt3lToPI6_s7Wn-2bZzLfde_v7hO1bOnkpz8H1w237F3Nt0BdtPmbkpDWPm5gE5KL0QGhtIPSStbH5I7pm5pJtDcvcsB59h84hMDMJoE2G0RhitEUYRYRQBQjXCKCKMaoTRCmF0aNMtwh6T0ev-TW9gldM4LAlWXGEx5XDBwhB2MYj5TAWqy2QUTIUKReYqhhXRHvPTAONiQFExBWXih0wKX6YSuHhE9uf5PDsmNHKmcAdzBReSBVIKAQtLv6uUM1WpCNrE21IvWZimK4lJlvCSXZonQPOkpHmbHAGhk_QDaMfk_BKLR1Fl-d02Oa4vjK5d_bI-itB1aZMXmi3VY9LlR0x1DP1kMJ4kg-DNIBxznsAapzt8q75QgufJH66fkPum8TEG756S_WK5zk7BnC3EMw23H29MogM |
link.rule.ids | 315,786,790,4043,27954,27955,27956 |
linkProvider | Flying Publisher |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Pectin+depolymerase+activities+associated+with+cell+walls+from+Cicer+arietinum+L.+epicotyl&rft.jtitle=Plant+and+cell+physiology&rft.au=SEVILLANO%2C+J.+M&rft.au=ZARRA%2C+I&rft.au=ACEBES%2C+J.+L&rft.date=1997&rft.pub=Oxford+University+Press&rft.issn=0032-0781&rft.eissn=1471-9053&rft.volume=38&rft.issue=11&rft.spage=1259&rft.epage=1263&rft_id=info:doi/10.1093%2Foxfordjournals.pcp.a029113&rft.externalDBID=n%2Fa&rft.externalDocID=2147593 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0032-0781&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0032-0781&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0032-0781&client=summon |