Pectin depolymerase [Pectic enzymes] activities associated with cell walls from Cicer arietinum L. epicotyl

• Published in: Plant and cell physiology Vol. 38; no. 11; pp. 1259 - 1263
• Main Authors: Sevillano, J.M. (Universidad de Leon (Spain)), Zarra, I, Acebes, J.L
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 1997
• Subjects: Arabinogalactan; arabinose; Biological and medical sciences; Cell wall; cell wall components; CELL WALLS; CICER ARIETINUM; enzyme activity; Enzymes; epicotyls; Fundamental and applied biological sciences. Psychology; galactans; galactose; GLICOSIDASAS; GLYCOSIDASE; GLYCOSIDASES; hydrolysis; Metabolism; PARED CELULAR; PAROI CELLULAIRE; Pectin; Pectin-depolymerase; PECTINAS; PECTINE; PECTINS; Plant physiology and development; polygalacturonase; polymerization; Polyuronide; polyuronides; protein composition; rhamnose; uronic acids; Enzyme; Pectin; Polygalacturonase; Cell wall; Hydrolysis; Leguminosae; Enzymatic activity; Cicer arietinum; Dicotyledones; Glycosidases; Angiospermae; Hydrolases; Isolation; Spermatophyta; O-Glycosidases; Polysaccharide
• ISSN: 0032-0781; 1471-9053
• DOI: 10.1093/oxfordjournals.pcp.a029113

The hydrolytic activity of the proteins extracted with 3 M LiCl from chick-pea (Cicer arietinum) cell walls to pectic fractions extracted with 50 mM trans-1,2-diaminocyclohexane-N,N,N',N'-tetraacetic acid (CDTA) and 50 mM sodium carbonate was studied. The pectic fractions contained acidic polysaccharides with high molecular mass (higher than 5 x 10(3) kDa), mainly composed of uronic acids, galactose, arabinose and rhamnose. The extracted proteins depolymerized the pectic polysaccharides and also a commercial preparation of polygalacturonic acid from citrus, detected by a decrease in their viscosity and a shift of their molecular mass distribution. The extract was able to depolymerize a uronic acid-rich component in all the cases, although in different extent. Also, with regard to the CDTA-soluble pectins, a degradation of polyuronide and a shift of the molecular mass distribution of arabinogalactan was observed