Pectin depolymerase [Pectic enzymes] activities associated with cell walls from Cicer arietinum L. epicotyl
The hydrolytic activity of the proteins extracted with 3 M LiCl from chick-pea (Cicer arietinum) cell walls to pectic fractions extracted with 50 mM trans-1,2-diaminocyclohexane-N,N,N',N'-tetraacetic acid (CDTA) and 50 mM sodium carbonate was studied. The pectic fractions contained acidic...
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Published in | Plant and cell physiology Vol. 38; no. 11; pp. 1259 - 1263 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Oxford
Oxford University Press
1997
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Subjects | |
Online Access | Get full text |
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Summary: | The hydrolytic activity of the proteins extracted with 3 M LiCl from chick-pea (Cicer arietinum) cell walls to pectic fractions extracted with 50 mM trans-1,2-diaminocyclohexane-N,N,N',N'-tetraacetic acid (CDTA) and 50 mM sodium carbonate was studied. The pectic fractions contained acidic polysaccharides with high molecular mass (higher than 5 x 10(3) kDa), mainly composed of uronic acids, galactose, arabinose and rhamnose. The extracted proteins depolymerized the pectic polysaccharides and also a commercial preparation of polygalacturonic acid from citrus, detected by a decrease in their viscosity and a shift of their molecular mass distribution. The extract was able to depolymerize a uronic acid-rich component in all the cases, although in different extent. Also, with regard to the CDTA-soluble pectins, a degradation of polyuronide and a shift of the molecular mass distribution of arabinogalactan was observed |
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Bibliography: | 1998003151 F60 istex:699AC0AF83B2DD13979A390B2808AA5EBA3E59D8 3 Author for correspondence. ArticleID:38.11.1259 ark:/67375/HXZ-H6GH7X99-1 |
ISSN: | 0032-0781 1471-9053 |
DOI: | 10.1093/oxfordjournals.pcp.a029113 |