Correlation of testicular toxicity and oxidative stress induced by chlorpyrifos in rats

• Published in: Human & experimental toxicology Vol. 30; no. 10; pp. 1529 - 1539
• Main Authors: Mandal, Tapas Kumar, Das, Niladri Sekhar
• Format: Journal Article
• Language: English
• Published: London, England SAGE Publications 01.10.2011; Sage Publications; Sage Publications Ltd
• Subjects: Animals; Biological and medical sciences; Chlorpyrifos - toxicity; Correlation analysis; Follicle Stimulating Hormone - blood; Insecticides - toxicity; Lipid Peroxidation; Luteinizing Hormone - blood; Male; Males; Malondialdehyde - metabolism; Medical sciences; Oxidative Stress; Oxidoreductases - metabolism; Pesticides; Pesticides, fertilizers and other agrochemicals toxicology; Rats; Rats, Sprague-Dawley; Reproductive system; Rodents; Spermatogenesis - drug effects; Testis - drug effects; Testis - metabolism; Testis - pathology; Testosterone - blood; Toxicity; Toxicology; testicular toxicity; Sprague-Dawley rats; chlorpyrifos; lipid peroxidation; oxidative stress; Chlorpyrifos; Oxidative stress; Correlation; Insecticide; Rat; Toxicity; Pesticides; Rodentia; Lipids; Testicle; Male genital system; Vertebrata; Mammalia; Animal; Organophosphorus compounds; Peroxidation
• ISSN: 0960-3271; 1477-0903
• DOI: 10.1177/0960327110392400

Effect of chlorpyrifos pesticide on testicular oxidative damage was studied in Sprague-Dawley rats at varying doses. At lower doses (5 and 10 mg/kg body weight/30 days), reduction in plasma levels of testosterone and follicular stimulating hormone (FSH) and luteinizing hormone (LH) along with significant shrinkage of seminiferous tubules and drastic changes in germ cells were seen. But these adverse changes of testes were restored with the revival of serum testosterone and FSH and LH at higher doses (20 and 30 mg/kg body weight/30 days). Similarly, levels of testicular lipid peroxidation and diene conjugates were elevated whereas activities of antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase), steroidogenic (Δ5, 3β-HSD and Δ5, 17β-HSD) enzymes and angiotensinogen-converting enzyme and glutathione content including lipid–protein content of testes were decreased at low doses. But at higher doses, reductions in level of lipid peroxidation (as revealed by malondialdehyde [MDA] value) and conjugated dienes were found and on the contrary, revivals of testicular antiperoxidative/antioxidant enzymes defense systems, angiotensinogen-converting enzyme (ACE), steroidogenic enzymes, lipid–protein and antioxidant glutathione content were observed. Therefore, the present study indicated from the results that chlorpyrifos had a dual effect at both doses on oxidative stress changes, but at higher doses, the cells were triggering its natural defense mechanism to combat the insult of lower doses of chlorpyrifos and became operative possibly through corrective measure of antioxidant enzymes defense system and pituitary gonadotropins hormones feedback mechanisms on testes.