Inhibition of Na currents by the toxin extracts from puffer fishes captured in the sea coast of Japan

• Published in: Toxicon (Oxford) Vol. 56; no. 6; pp. 999 - 1006
• Main Authors: Iwata, Satomi, Matsuura, Tatsuya, Yamamoto, Sokatsu, Tahara, Tatsuo, Shin, Min-Chul, Ito, Yushi, Akaike, Norio
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.11.2010; Elsevier
• Subjects: Animal poisons toxicology. Antivenoms; Animals; Biological and medical sciences; Cells, Cultured; Coastal environments; Edible; Fish; Hippocampal CA1 neuron; Hippocampus - cytology; Inhibition; Inhibitory Concentration 50; Japan; Liver; Liver - chemistry; Marine; Medical sciences; Muscle, Skeletal - chemistry; Muscles; Neurons; Neurons - drug effects; Neurons - metabolism; Patch-Clamp Techniques; Puffer fishes; Rats; Rats, Wistar; Sodium; Sodium Channel Blockers - toxicity; Sodium Channels - drug effects; Sodium Channels - metabolism; Sodium current; Species Specificity; Tetraodontiformes - physiology; Tetrodotoxin; Tetrodotoxin - toxicity; Tissue Extracts - toxicity; Toxicology; Toxin extract; Toxins; Asia; Japan; Tetrodotoxin; Puffer fishes; Hippocampal CA1 neuron; Toxin extract; Sodium current; Central nervous system; Extract; Encephalon; Marine environment; Toxin; Vertebrata; Neuron; Sodium; Pisces; Sodium ion; Inhibition; Hippocampus
• ISSN: 0041-0101; 1879-3150
• DOI: 10.1016/j.toxicon.2010.07.004

The inhibitory effects of toxin extracted from muscle or liver of five different puffer fishes (hereafter referred as puffer(s)) captured on the Japanese sea coast were examined on voltage-dependent sodium current ( I Na) recorded from dissociated single rat hippocampal CA1 neurons. The inhibitory effects estimated from IC 50 values of toxin extracts on I Na were in the order of Takifugu vermicularis >  Lagocephalus wheeleri >  Canthigaster rivulata >  Takifugu rubripes >  Arothron reticularis from muscle and T. vermicularis >  T. rubripes >  L. wheeleri >  A. reticularis >  C. rivulata from liver, thereby indicating that the amount of toxin in the liver or muscle differs between puffers. In addition, the present results indicate that the muscle of T. vermicularis, which is eaten in Japan, contains relatively higher amounts of toxin compared to those of T. rubripes, also eaten. This observation suggests that caution should be taken concerning the maximal edible amount of muscle prepared from T. rubripes.