Determination of Synthetic Hormones in Animal Urine by High-Performance Liquid Chromatography/Mass Spectrometry

• Published in: Journal of AOAC International Vol. 90; no. 2; pp. 626 - 632
• Main Authors: Rubies, A, Cabrera, A, Centrich, F
• Format: Journal Article
• Language: English
• Published: Gaithersburg, MD AOAC International 01.03.2007; Oxford University Press
• Subjects: Animals; Biological and medical sciences; Calibration; Cattle; Chemistry Techniques, Analytical - methods; Chromatography; Chromatography, Liquid - methods; Common Agricultural Policy; drug residues; farmed animal species; food animals; Food industries; Fundamental and applied biological sciences. Psychology; growth promotion; High performance liquid chromatography; hormone supplements; Hormones - analysis; Hormones - urine; Hormones in animal nutrition; Hydrolysis; Identification and classification; illegal veterinary drug; Mass spectrometry; Mass Spectrometry - methods; Methods; quantitative analysis; Reproducibility of Results; Sheep; somatotropin; Swine; synthetic products; Temperature; Time Factors; Urinalysis - methods; urine; veterinary drugs; veterinary medicine; zeranol; Spain; Hormone; Urine; HPLC chromatography; Biological fluid; Mass spectrometry; Animal
• ISSN: 1060-3271; 1944-7922
• DOI: 10.1093/jaoac/90.2.626

A method was developed for the extraction of stanozolol, taleranol, zeranol, hexestrol, dienestrol, ethynylestradiol, diethylstilbestrol, and trenbolone from animal urine. The analytes were extracted from the matrix by enzymatic hydrolysis, solid-phase extraction, and liquid-liquid extraction. Detection and quantitation were performed on a high-performance liquid chromatography system coupled to a triple quadrupole mass spectrometer. The identification criteria met the European Union regulations. Validation of this method established a decision limit between 0.2 and 0.9 μg/L and a detection capability between 0.3 and 1.0 μg/L, depending on the analyte.