Chemiluminescence evaluation of oxidative damage to biomolecules induced by singlet oxygen and the protective effects of antioxidants

• Published in: Biochimica et biophysica acta Vol. 1725; no. 1; pp. 30 - 34
• Main Authors: Wenli, Yu, Yaping, Zhao
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 30.08.2005
• Subjects: Antioxidant; Antioxidants - chemistry; Chemiluminescence; DNA - chemistry; Linoleic Acid - chemistry; Luminescent Measurements; Oxidation-Reduction; Oxidative damage; Oxidative Stress; Protective Agents - chemistry; Singlet oxygen; Singlet Oxygen - chemistry; Chemiluminescence; Singlet oxygen; Antioxidant; Oxidative damage
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/j.bbagen.2005.05.002

A chemiluminescence (CL) method was developed for the evaluation of oxidative damage to biomolecules induced by singlet oxygen ( 1O 2) and for the evaluation of the protective effects of antioxidants. The 1O 2 was generated from the reaction of H 2O 2 + OCl −. Results showed that the CL signal from the reaction of H 2O 2 + OCl − was weak, however, it was enhanced dose-dependently with the addition of DNA and unsaturated fatty acid, respectively. Spectra analysis indicated that the enhanced CL could be ascribed to the decay of triplet-excited carbonyl compounds, which were generated from the reaction of 1O 2 plus the biomolecules. On the other hand, the enhanced CL produced in the above systems could be effectively inhibited by lycopene, β-carotene, VC, and VE, but could not be inhibited by mannitol, SOD, and NaN 3. The mechanism therein was discussed.