Incorporation of conjugated linoleic acid isomers into porcine erythrocytes

• Published in: European journal of nutrition Vol. 53; no. 3; pp. 989 - 993
• Main Authors: Malovrh, Tomaž, Melkić, Enver, Kompan, Drago, Levart, Alenka, Kompan, Lidija
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.04.2014; Springer Berlin Heidelberg; Springer; Springer Nature B.V
• Subjects: Animals; Animals, Inbred Strains; Biological and medical sciences; Biological Transport; cell membranes; Chemistry; Chemistry and Materials Science; conjugated linoleic acid; Diet - veterinary; Dietary Fats, Unsaturated - metabolism; Energy Intake; Erythrocyte Membrane - metabolism; erythrocytes; Feeding. Feeding behavior; Fundamental and applied biological sciences. Psychology; isomers; Kinetics; Linoleic Acids, Conjugated - metabolism; Nutrition; piglets; Plant Oils - metabolism; Short Communication; Slovenia; Sunflower Oil; Sus scrofa - blood; Sus scrofa - growth & development; Sus scrofa - physiology; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Weight Gain; Conjugated linoleic acid; Fatty acid methyl esters; Porcine erythrocytes; Nutrition; Red blood cell; Isomer; Pig; Blood cell; Vertebrata; Mammalia; Animal; Artiodactyla; Fatty acid methyl ester; Ungulata
• ISSN: 1436-6207; 1436-6215
• DOI: 10.1007/s00394-013-0618-3

PURPOSE: The aim of the current study was to determine the incorporation of cis (c) 9, trans (t) 11-conjugated linoleic acid (CLA) and t10, c12-CLA into porcine erythrocytes—both isomers were supplemented in equal proportions. METHODS: The study group consisted of 16 piglets randomly assigned into experimental and control group. For the period of 5 weeks, the piglets from the experimental group were receiving a 1.2 % CLA supplement while the controls were supplemented with the same amount of sunflower oil. For the remaining 7 weeks, the piglets were fed without a supplement. Blood samples to evaluate incorporation of CLA into erythrocyte membranes were taken from all animals on weekly basis. RESULTS: Compared to t10, c12-CLA isomer, proportion of c9, t11-CLA isomer in the membrane of erythrocytes was higher for the whole time of the study period. After 4 weeks of feeding, it approaches the plateau. The peak value for both isomers was measured at the end of week 5, with a value of 3.24 g c9, t11-CLA/100 g of fatty acids and a 1.09 g t10, c12-CLA/100 g of fatty acids (p < 0.0001). After cessation of supplementation, the proportion of both isomers gradually decreased to be almost completely washed out—in 7 weeks. CONCLUSIONS: During supplementation with equivalent amounts of CLA isomers, their proportion in membranes of porcine erythrocytes increases with time, with higher proportion of c9, t11-CLA. CLA isomers probably differently incorporate into different cell membranes at different species which could explain its various biological functions.