Novel Hepatitis B Virus Subgenotype in the Southern Yunnan Province of China

Hepatitis B virus (HBV) infection is highly prevalent in China. To identify the genotypes of HBV in the southern Yunnan Province of China, full-length HBV genomes were extracted from 1 Dai and 4 Hani HBV carriers and linked with the pMD T-18 vector. For each patient, 3–10 clones were sequenced direc...

Full description

Saved in:
Bibliographic Details
Published inIntervirology Vol. 52; no. 6; pp. 340 - 346
Main Authors Shen, Tao, Gao, Jian-mei, Zou, Yun-Lian, Dong, Hong, Yan, Xin-Min
Format Journal Article
LanguageEnglish
Published Basel, Switzerland S. Karger AG 01.01.2009
Subjects
Adolescent
Adult
China - epidemiology
Consensus Sequence
DNA, Viral - genetics
DNA, Viral - isolation & purification
Evolution, Molecular
Female
Genetic Variation
Genome, Viral
Hepatitis B
Hepatitis B - epidemiology
Hepatitis B - virology
Hepatitis B virus
Hepatitis B virus - genetics
Humans
Male
Molecular Sequence Data
Phylogeny
Sequence Analysis, DNA
Short Communication
China
Hepatitis B virus
Phylogenetic tree
Genotypes
Subgenotypes
Online AccessGet full text

Cover

More Information
Summary:Hepatitis B virus (HBV) infection is highly prevalent in China. To identify the genotypes of HBV in the southern Yunnan Province of China, full-length HBV genomes were extracted from 1 Dai and 4 Hani HBV carriers and linked with the pMD T-18 vector. For each patient, 3–10 clones were sequenced directly and a consensus sequence was created. Genotypic and serotypic analysis revealed 4 HBV/B (2 B2 with adw2 and 2 new subgenotypes with ayw1) and 1 HBV/C (C1 with adrq+) genotypes. The divergences of the entire genome sequences of the new subgenotype were 0–0.9% and 2.99–6.48% between other known HBV/B. Divergences in other coding regions revealed that it was more similar to B3 and B4 in the precore/core gene (2.02 and 2.09%, respectively), and similar to B3 and B5 in the preS1/S2/S gene (2.24 and 2.78%, respectively). Phylogenetic trees using the precore/core and X genes both revealed a new clad separating from the major trunk of genotype B with a 99% bootstrap value. These results show that the 2 consensus isolates are a mosaic of B3–B5, which we designated to subgenotype B6. Considering the geographical distances, the relationship between B6 and other HBV/B subgenotypes (B3–B5) and HBV evolution needs to be further studied.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
ISSN:0300-5526
1423-0100
DOI:10.1159/000252912