The monomeric photosystem I-complex of the diatom Phaeodactylum tricornutum binds specific fucoxanthin chlorophyll proteins (FCPs) as light-harvesting complexes

A photosystem I (PSI)–fucoxanthin chlorophyll protein (FCP) complex with a chlorophyll a/P700 ratio of approximately 200:1 was isolated from the diatom Phaeodactylum tricornutum. Spectroscopic analysis proved that the more tightly bound FCP functions as a light-harvesting complex, actively transferr...

Full description

Saved in:
Bibliographic Details
Published inBiochimica et biophysica acta Vol. 1767; no. 12; pp. 1428 - 1435
Main Authors Veith, Thomas, Büchel, Claudia
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.12.2007
Subjects
Online AccessGet full text

Cover

Loading…
More Information
Summary:A photosystem I (PSI)–fucoxanthin chlorophyll protein (FCP) complex with a chlorophyll a/P700 ratio of approximately 200:1 was isolated from the diatom Phaeodactylum tricornutum. Spectroscopic analysis proved that the more tightly bound FCP functions as a light-harvesting complex, actively transferring light energy from its accessory pigments chlorophyll c and fucoxanthin to the PSI core. Using an antibody against all FCP polypeptides of Cyclotella cryptica it could be shown that the polypeptides of the major FCP fraction differ from the FCPs found in the PSI fraction. Since these FCPs are tightly bound to PSI, active in energy transfer, and not found in the main FCP fraction, we suppose them to be PSI specific. Blue Native-PAGE, gel filtration and first electron microscopy studies of the PSI–FCP sample revealed a monomeric complex comparable in size and shape to the PSI–LHCI complex of green algae.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0005-2728
0006-3002
1879-2650
DOI:10.1016/j.bbabio.2007.09.004