Association of angiotensin-converting enzyme (ACE) I/D variation with biochemical parameters and oxidative stress markers in systemic lupus erythematosus patients in west of Iran
Purpose We aimed to study insertion/deletion (I/D) variation (rs4646994) of ACE gene in a group of SLE patients in west of Iran and its possible relationship with oxidative stress. Method and results Genotypes and allele frequencies related to ACE (I/D) variation were determined in 108 SLE patients...
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Published in | Molecular biology reports Vol. 50; no. 10; pp. 8201 - 8212 |
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Main Authors | , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer Netherlands
01.10.2023
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Purpose
We aimed to study insertion/deletion (I/D) variation (rs4646994) of
ACE
gene in a group of SLE patients in west of Iran and its possible relationship with oxidative stress.
Method and results
Genotypes and allele frequencies related to
ACE
(I/D) variation were determined in 108 SLE patients and 110 gender and age-matched healthy controls using PCR. Neopterin, malondialdehyde (MDA), and serum lipid concentrations were determined by HPLC and enzyme assay respectively. The overall distribution of
ACE
I/D genotypes in SLE patients was different from that of the control group (P = 0.005). DD genotype compared to ID genotype increased the risk of SLE (OR = 2.57, 95% CI 1.4–4.8, P = 0.003). ID genotype compared to the II genotype decreased the risk of disease (OR = 0.45, 95% CI 0.2–0.99, p = 0.042). SLE patients with DD, ID, and II genotypes had lower paraoxonase (PON) activity and higher serum levels of MDA and neopterin versus control patients. We also detected a significant protective effect against SLE in presence of
ACE
I alleles and lack of angiotensin II receptor, type 1 (
AGTR1
) A1166C (NCBI reference SNP id: rs5186), C alleles in this study (OR = 0.31, 95% CI 0.14–0.68, P = 0.002).
Conclusions
Carriers of the DD genotype of ACE gene with higher serum concentrations of neopterin and MDA, and lower PON activity had a high risk to develop SLE, while ID genotype decreased the risk of disease development by 2.22 times compared to II genotype. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0301-4851 1573-4978 1573-4978 |
DOI: | 10.1007/s11033-023-08685-x |