Comparison of the Surface Coat Proteins of the Pine Wood Nematode Appeared During Host Pine Infection and In Vitro Culture by a Proteomic Approach

• Published in: Phytopathology Vol. 100; no. 12; pp. 1289 - 1297
• Main Authors: SHINYA, Ryoji, MORISAKA, Hironobu, TAKEUCHI, Yuko, UEDA, Mitsuyoshi, FUTAI, Kazuyoshi
• Format: Journal Article
• Language: English
• Published: St. Paul, MN American Phytopathological Society 01.12.2010
• Subjects: Animals; Biological and medical sciences; Botrytis - physiology; Botrytis cinerea; Bursaphelenchus xylophilus; Chromatography, High Pressure Liquid; Fundamental and applied biological sciences. Psychology; Helminth Proteins - genetics; Helminth Proteins - isolation & purification; Microscopy, Electron, Scanning; Nematoda; Nematoda - genetics; Nematoda - isolation & purification; Nematoda - ultrastructure; Phytopathology. Animal pests. Plant and forest protection; Pinus - microbiology; Pinus - parasitology; Plant Diseases - microbiology; Proteomics - methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Plant pathology; Plant; Plant pathogen; In vitro; Protein; Proteomics
• ISSN: 0031-949X; 1943-7684
• DOI: 10.1094/phyto-04-10-0109

Pine wilt disease, caused by the pine wood nematode (PWN), Bursaphelenchus xylophilus, has become of worldwide quarantine concern in recent years. Here, we disclosed the surface coat (SC) proteins of the PWN which are thought to be one of the key components in pine wilt development. This is the first report that focused on the SC proteins and thoroughly identified those proteins of a plant-parasitic nematode using the proteomic approach. In this study, SC protein profiles were compared for PWNs grown on the fungus Botrytis cinerea and in host pine seedlings. The results demonstrated that the gross amount of PWN SC proteins drastically increased during infection of the host pine. Thirty-seven protein bands showed significant quantity differences between fungus-grown and host-origin PWNs, and were used for identification by matrix-assisted laser desorption ionization time of flight mass spectrometry analysis. These included several proteins that are presumed to be involved in the host immune response; for example, regulators of reactive oxygen species (ROS) and a ROS scavenger. These results might suggest that the PWN SC proteins are crucial in modulating or evading host immune response. Our data provide a new insight into the mechanism of pine wilt disease and the biological role of the SC proteins of plant-parasitic nematodes.