Development of microsatellite markers in Capsella rubella and Capsella bursa‐pastoris (Brassicaceae)

• Premise of the study: We developed microsatellite markers to investigate genetic diversity within and among populations of Capsella rubella and Capsella bursa‐pastoris and between these two species. • Methods and Results: Fourteen polymorphic microsatellite loci were identified in the two species...

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Bibliographic Details
Published inAmerican journal of botany Vol. 98; no. 7; pp. e176 - e179
Main Authors Caullet, Coraline M. L., Pernin, Fanny, Poncet, Charles, Le Corre, Valérie
Format Journal Article
LanguageEnglish
Published United States Botanical Society of America 01.07.2011
Botanical Society of America, Inc
Subjects
Brassicaceae
Brassicaceae - genetics
Capsella - genetics
Capsella bursa-pastoris
Capsella rubella
DNA Primers - genetics
Europe
Flowers & plants
Genetic diversity
Genetic Loci - genetics
Genetic Techniques
Genetic Testing
Genetics, Population
Genotype & phenotype
Geography
microsatellite markers
Microsatellite Repeats - genetics
Plant populations
Polymorphism
Europe
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Summary:• Premise of the study: We developed microsatellite markers to investigate genetic diversity within and among populations of Capsella rubella and Capsella bursa‐pastoris and between these two species. • Methods and Results: Fourteen polymorphic microsatellite loci were identified in the two species and one more polymorphic microsatellite locus only in C. rubella. Samples from different European localities were genotyped. Up to six alleles per locus were observed in C. rubella, and up to 22 alleles per locus in C. bursa‐pastoris. Observed heterozygosities were low, indicating high selfing rates, especially in C. rubella. • Conclusions: The results provide valuable information on genetic diversity for future studies of population genetics in C. rubella and C. bursa‐pastoris.
Bibliography:C.M.L.C. benefited from a grant from the French Ministry of Higher Education and Research.
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ISSN:0002-9122
1537-2197
DOI:10.3732/ajb.1100081