Simple and rapid high-pressure liquid chromatographic determination of papaverine in plasma

A rapid and sensitive high-pressure liquid chromatographic (HPLC) assay is described for the quantification of papaverine in plasma. A paired-ion, reversed-phase system was employed with detection at 254 nm. This procedure yielded reproducible results with a sensitivity of 2 ng/ml. Total extraction...

Full description

Saved in:
Bibliographic Details
Published inJournal of pharmaceutical sciences Vol. 68; no. 12; p. 1550
Main Authors Pierson, S L, Hanigan, J J, Taylor, R E, McClurg, J E
Format Journal Article
LanguageEnglish
Published United States 01.12.1979
Subjects
Biological Availability
Chromatography, High Pressure Liquid
Humans
Male
Methods
Papaverine - blood
Time Factors
Online AccessGet more information

Cover

More Information
Summary:A rapid and sensitive high-pressure liquid chromatographic (HPLC) assay is described for the quantification of papaverine in plasma. A paired-ion, reversed-phase system was employed with detection at 254 nm. This procedure yielded reproducible results with a sensitivity of 2 ng/ml. Total extraction and quantification time was less than 1 hr. The suitability of this procedure for plasma sample analysis from a bioavailability study was demonstrated.
ISSN:0022-3549
DOI:10.1002/jps.2600681224