Organization and regulation of the Corynebacterium glutamicum hom-thrB and thrC loci
The genes encoding the three terminal enzymes in the threonine biosynthetic pathway, homoserine dehydrogenase (hom), homoserine kinase (thrB) and threonine synthase (thrC) have been isolated from Corynebacterium glutamicum. The C. glutamicum hom and thrB genes were subcloned on a 3.6 kb SalI-generat...
Saved in:
Published in | Molecular microbiology Vol. 2; no. 1; p. 53 |
---|---|
Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
England
01.01.1988
|
Subjects | |
Online Access | Get more information |
Cover
Loading…
Summary: | The genes encoding the three terminal enzymes in the threonine biosynthetic pathway, homoserine dehydrogenase (hom), homoserine kinase (thrB) and threonine synthase (thrC) have been isolated from Corynebacterium glutamicum. The C. glutamicum hom and thrB genes were subcloned on a 3.6 kb SalI-generated chromosomal fragment. The C. glutamicum thrC gene was shown not to be linked to the hom-thrB locus. L-methionine represses the cloned homoserine dehydrogenase and homoserine kinase similar to that of the chromosomally encoded hom and thrB gene products. Northern hybridization analysis demonstrates that this repression is mediated at the level of transcription and that hom-thrB represents an operon in C. glutamicum. |
---|---|
ISSN: | 0950-382X |
DOI: | 10.1111/j.1365-2958.1988.tb00006.x |