Organization and regulation of the Corynebacterium glutamicum hom-thrB and thrC loci

The genes encoding the three terminal enzymes in the threonine biosynthetic pathway, homoserine dehydrogenase (hom), homoserine kinase (thrB) and threonine synthase (thrC) have been isolated from Corynebacterium glutamicum. The C. glutamicum hom and thrB genes were subcloned on a 3.6 kb SalI-generat...

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Published inMolecular microbiology Vol. 2; no. 1; p. 53
Main Authors Follettie, M T, Shin, H K, Sinskey, A J
Format Journal Article
LanguageEnglish
Published England 01.01.1988
Subjects
Alcohol Oxidoreductases - genetics
Carbon-Oxygen Lyases
Chromosomes, Bacterial - physiology
Corynebacterium - enzymology
Corynebacterium - genetics
DNA Restriction Enzymes
Genes
Genes, Bacterial
Genes, Regulator
Genotype
Homoserine Dehydrogenase - genetics
Lyases - genetics
Phosphotransferases (Alcohol Group Acceptor)
Phosphotransferases - genetics
Plasmids
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Summary:The genes encoding the three terminal enzymes in the threonine biosynthetic pathway, homoserine dehydrogenase (hom), homoserine kinase (thrB) and threonine synthase (thrC) have been isolated from Corynebacterium glutamicum. The C. glutamicum hom and thrB genes were subcloned on a 3.6 kb SalI-generated chromosomal fragment. The C. glutamicum thrC gene was shown not to be linked to the hom-thrB locus. L-methionine represses the cloned homoserine dehydrogenase and homoserine kinase similar to that of the chromosomally encoded hom and thrB gene products. Northern hybridization analysis demonstrates that this repression is mediated at the level of transcription and that hom-thrB represents an operon in C. glutamicum.
ISSN:0950-382X
DOI:10.1111/j.1365-2958.1988.tb00006.x