Early plasma biomarker dynamic profiles are associated with acute ischemic stroke outcomes

• Published in: European journal of neurology Vol. 29; no. 6; pp. 1630 - 1642
• Main Authors: Correia, Manuel, Silva, Isabel, Gabriel, Denis, Simrén, Joel, Carneiro, Angelo, Ribeiro, Sara, Dória, Hugo Mota, Varela, Ricardo, Aires, Ana, Minta, Karolina, Antunes, Rui, Felgueiras, Rui, Castro, Pedro, Blenow, Kaj, Magalhães, Rui, Zetterberg, Henrik, Maia, Luis F.
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.06.2022
• Subjects: Biomarkers; blood; Blood vessels; brevican; early ct score; fibrillary acidic protein; GFAP; Glial Fibrillary Acidic Protein; Humans; injury; Ischemia; Ischemic Stroke; Marker panels; Medical imaging; neurofilament light-chain; Neuroimaging; Neuronal-glial interactions; Neurosciences; Neurosciences & Neurology; Neurovetenskaper; NfL; Occlusion; outcome; Patients; plasma biomarkers; Prospective Studies; Stroke; Stroke - diagnostic imaging; Stroke - surgery; Tau protein; Thrombectomy; total-tau; NfL; thrombectomy; plasma biomarkers; total-tau; stroke; outcome; GFAP
• ISSN: 1351-5101; 1468-1331
• DOI: 10.1111/ene.15273

Background Early outcome prediction after acute ischemic stroke (AIS) might be improved with blood‐based biomarkers. We investigated whether the longitudinal profile of a multi‐marker panel could predict the outcome of successfully recanalized AIS patients. Methods We used ultrasensitive single‐molecule array (Simoa) to measure glial fibrillary acidic protein (GFAP), neurofilament light chain (NfL), total‐tau (t‐tau) and ELISA for brevican in a prospective study of AIS patients with anterior circulation large vessel occlusion successfully submitted to thrombectomy. Plasma was obtained at admission, upon treatment, 24 h and 72 h after treatment. Clinical and neuroimaging outcomes were assessed independently. Results Thirty‐five patients (64.8%) had good early clinical or neuroimaging outcome. Baseline biomarker levels did not distinguish between outcomes. However, longitudinal intra‐individual biomarker changes followed different dynamic profiles with time and according to outcome. GFAP levels exhibited an early and prominent increase between admission and just after treatment. NfL increase was less pronounced between admission and up to 24 h. T‐tau increased between treatment and 24 h. Interestingly, GFAP rate‐of‐change (pg/ml/h) between admission and immediately after recanalization had a good discriminative capacity between clinical outcomes (AUC = 0.88, p < 0.001), which was higher than admission CT‐ASPECTS (AUC = 0.75, p < 0.01). T‐tau rate‐of‐change provided moderate discriminative capacity (AUC = 0.71, p < 0.05). Moreover, in AIS patients with admission CT‐ASPECTS <9 both GFAP and NfL rate‐of‐change were good outcome predictors (AUC = 0.82 and 0.77, p < 0.05). Conclusion Early GFAP, t‐tau and NfL rate‐of‐change in plasma can predict AIS clinical and neuroimaging outcome after successful recanalization. Such dynamic measures match and anticipate neuroimaging predictive capacity, potentially improving AIS patient stratification for treatment, and targeting individualized stroke care. Longitudinal biomarker panel profile in the acute phase of stroke patient care.