Essential role of w/o microemulsion structure on catalytic activity of entrapped proteins studied by small-angle X-ray scattering and circular dichroism

For understanding the mechanism of enhancement of catalytic activity of proteins in w/o microemulsions, it is very important to clarify a relation between structural characteristics of w/o microemulsion and occluded protein. We have studied the structure and catalytic activity of two different types...

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Bibliographic Details
Published inJournal of applied crystallography Vol. 36; no. 3-1; pp. 530 - 534
Main Authors Kawai-Hirai, Rika, Hirai, Mitsuhiro
Format Journal Article Conference Proceeding
LanguageEnglish
Published 5 Abbey Square, Chester, Cheshire CH1 2HU, England Munksgaard International Publishers 01.06.2003
Blackwell
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Summary:For understanding the mechanism of enhancement of catalytic activity of proteins in w/o microemulsions, it is very important to clarify a relation between structural characteristics of w/o microemulsion and occluded protein. We have studied the structure and catalytic activity of two different types of bovine serum albumins occluded in biological solvent / sodium bis(2‐ethylhexyl)sulfosuccinate (AOT) / 2,2,4‐trimethylpentane (isooctane) microemulsions. The secondary structures of the albumins both in AOT w/o microemulsions and in aqueous buffer solvents containing AOT have been measured by using circular dichroism. The structures of AOT microemulsions occluding the proteins have been studied by using synchrotron radiation small‐angle X‐ray scattering. We will show the detailed relation between the microemulsion structure and the catalytic activity of the proteins.
Bibliography:istex:0F59FB640FD20D9C5C929B3CDFFE958D6B00CAE8
ark:/67375/WNG-PV7NQ9ZP-H
ArticleID:JCRKS7413
ISSN:1600-5767
0021-8898
1600-5767
DOI:10.1107/S0021889803003832