Photosensitization of the rat endometrium following 5-aminolevulinic acid induced photodynamic therapy
Background and Objective The impact of photodynamic therapy (PDT) on the endometrium following topical application of 5‐aminolevulinic acid (ALA) was studied in a rat model. Study Design/Materials and Methods Fluorescence microscopy revealed peak ALA to protoporphyrin IX (Pp IX) conversion 3–6 hours...
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Published in | Lasers in surgery and medicine Vol. 18; no. 3; pp. 301 - 308 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
New York
John Wiley & Sons, Inc
1996
Wiley-Liss |
Subjects | |
Online Access | Get full text |
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Summary: | Background and Objective
The impact of photodynamic therapy (PDT) on the endometrium following topical application of 5‐aminolevulinic acid (ALA) was studied in a rat model.
Study Design/Materials and Methods
Fluorescence microscopy revealed peak ALA to protoporphyrin IX (Pp IX) conversion 3–6 hours after application. Significantly higher Pp IX levels were observed in the endometrial glands compared with endometrial stroma and myometrium.
Results
Histological studies showed PDT‐induced endometrial destruction with atrophy 7–10 weeks after treatment. Reproductive performance studies demonstrated significant implantation failure in the treated uterine horns compared with controls. The number of implantation sacs in the treated and untreated horns was 0.4 ± 0.3 and 8.9 ± 1.0, respectively (P < 0.01).
Conclusion
We conclude that the high rate of Pp IX conversion in the endometrial glands can be exploited to treat dysfunctional uterine bleeding with PDT. In addition, this concept may also be applied to study endometrial regeneration and embryo implantation mechanisms. © 1996 Wiley‐Liss, Inc. |
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Bibliography: | ArticleID:LSM12 ark:/67375/WNG-ZV0JTKMZ-1 istex:3D5A77A6F17C2C68FCAE5D21F8767C18129D3DDE NIH - No. 2R01 CA32248; No. 5P41 RR01192; No. DOE DE-FG03-91ER61227; No. ONR N00014-91-C-0134 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0196-8092 1096-9101 |
DOI: | 10.1002/(SICI)1096-9101(1996)18:3<301::AID-LSM12>3.0.CO;2-8 |