Induction of gamma-radiation of DNA synthesis in radicle cells of germinating seeds of Pisum sativum L

In radicle meristem cells of germinating seeds of the pea (Pisum sativum L) before the onset of replicative synthesis of DNA, irradiation with 2-3 krad of gamma-rays induced the incorporation of 3H-thymidine (3H-TdR). Maximum isotope incorporation was noted during the first 2 hours after irradiation...

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Published inInternational journal of radiation biology and related studies in physics, chemistry and medicine Vol. 29; no. 5; pp. 455 - 462
Main Authors Gudkov, I.N, Grodzinsky, D.M
Format Journal Article
LanguageEnglish
Published England 01.01.1976
Subjects
Acriflavine - pharmacology
Caffeine - pharmacology
Cobalt Radioisotopes
DNA Repair
DNA Replication - drug effects
Gamma Rays
Hydroxyurea - pharmacology
peas
Radiation Genetics
Seeds - drug effects
Seeds - radiation effects
Uracil - analogs & derivatives
Uracil - pharmacology
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Summary:In radicle meristem cells of germinating seeds of the pea (Pisum sativum L) before the onset of replicative synthesis of DNA, irradiation with 2-3 krad of gamma-rays induced the incorporation of 3H-thymidine (3H-TdR). Maximum isotope incorporation was noted during the first 2 hours after irradiation. Higher doses of radiation suppressed 3H-TdR incorporation. It was not seen after gamma-irradiation of air-dried seeds, nor after fast-neutron irradiation. The replication inhibitors hydroxyurea and 5-aminouracil had no effect on the gamma-induced incorporation of 3H-TdR, Whereas caffeine and acriflavine inhibited it to some extent. It is suggested that the gamma-radiation-induced incorporation of 3H-TdR in meristem cells during the pre-replicative period may be connected with repair phenomena.
ISSN:0020-7616
DOI:10.1080/09553007614550551