Ontogenetic changes in enzyme activities associated with energy production in the spiny lobster, Jasus edwardsii

• Published in: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology Vol. 130; no. 3; pp. 339 - 347
• Main Authors: Wells, R.M.G, Lu, J, Hickey, A.J.R, Jeffs, A.G
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 01.10.2001
• Subjects: Allometry; Animals; Body Weight; Development; Energy Metabolism; Enzyme; Enzymes - metabolism; Isoenzymes - metabolism; Jasus edwardsii; Larva - enzymology; Larva - growth & development; Larva - metabolism; Linear Models; Lobster; Marine; Metabolism; Nephropidae - enzymology; Nephropidae - growth & development; Nephropidae - metabolism; Phyllosome; Puerulus; Reproducibility of Results; Jasus edwardsii; Lobster; Enzyme; Development; Puerulus; Metabolism; Allometry; Phyllosome
• ISSN: 1096-4959; 1879-1107
• DOI: 10.1016/S1096-4959(01)00439-0

The larval life of the spiny lobster Jasus edwardsii is one of the longest and most complex of any marine organism and is poorly understood due to the difficulty of studying cryptic, pelagic organisms. Hence, the capacity for active swimming in the phyllosoma, puerulus and juvenile stages and the use of possible metabolic fuel reserves was inferred from a number of enzyme activities, including citrate synthase, lactate dehydrogenase, and HOAD. High activities of CS and LDH in abdominal tissues of Stage 11 phyllosoma and pueruli are consistent with a capacity to commence active on-shore movement. The activities of LDH and HOAD showed positive allometry while CS was independent of body mass. The body mass dependence of LDH activity may reflect the developing ability of the lobster to initiate brief escape manoeuvres, and the scaling of HOAD reflects an increased use of lipid fuel reserves. Aerobic enzyme activities were higher in abdominal tissues than in cephalic tissues of pelagic pueruli, but high activities appear in the cephalic tissues of juveniles. These changes mirror a developmental shift in activity from pelagic oceanic swimming to a benthic existence on the seabed of the near shore. The low LDH activity in pueruli confirmed previous findings that they have limited feeding capacity, with carbohydrate contributing little towards the major energy reserves. The highest LDH activities occur in the abdominal muscles of juveniles and correlate with rapid tail-flicking escape behaviour. The activities of HOAD increased throughout development, and in the abdominal tissues of juveniles, may reflect lipid transformation and accumulation as an energy reserve. Enzyme activities, therefore, provide useful information concerning migratory behaviour that is presently unavailable from ecological studies.