Characterisation and expression analysis of the chicken interleukin-7 receptor alpha chain
Interleukin-7 (IL-7) is a central regulator of T cell survival and homeostasis and its expression is indicative for naïve and memory T cells. We cloned chicken IL-7Rα (CHIL-7Rα) and determined its expression profile in chicken lymphocyte subpopulations. The predicted protein sequence contained 460 a...
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Published in | Developmental and comparative immunology Vol. 33; no. 9; pp. 1018 - 1026 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Ltd
01.09.2009
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Subjects | |
Online Access | Get full text |
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Summary: | Interleukin-7 (IL-7) is a central regulator of T cell survival and homeostasis and its expression is indicative for naïve and memory T cells. We cloned chicken IL-7Rα (CHIL-7Rα) and determined its expression profile in chicken lymphocyte subpopulations. The predicted protein sequence contained 460 amino acids. The extracellular domain exhibited features typical of a type I cytokine receptor; a fibronectin type III domain and the GXWSXWS motif were conserved. ChIL-7Rα mRNA is highly expressed in lymphoid organs and in CD4+, CD8α+ and CD8β+ cells. A monoclonal antibody was generated and expression of the protein investigated. ChIL-7Rα was expressed on CD4+ and CD8α+, but not CD8β+, T cells, in contrast to the high mRNA expression levels in all of these cells. Upon polyclonal stimulation with ConA, IL-7Rα was rapidly down-regulated on T cells, suggesting that in the chicken expression of this receptor might also be correlated to the T cell activation status. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0145-305X 1879-0089 |
DOI: | 10.1016/j.dci.2009.05.001 |