Synthesis of Poly-(R-hydroxyalkanoates) by Cupriavidus necator ATCC 17699 Using Mexican Avocado (Persea americana) Oil as a Carbon Source

Poly-R-hydroxyalkanoates (PHAs) are polymers produced by a vast number of bacterial species under stress conditions. PHAs exhibit different thermal and mechanical properties that depend on their molecular structure. In this work, PHAs were produced using avocado oil as the carbon source. Cupriavidus...

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Bibliographic Details
Published inInternational journal of polymer science Vol. 2017; no. 2017; pp. 1 - 10
Main Authors Martín-Bufájer, José Mauricio, Figueroa-López, Ulises, Pérez-Guevara, Fermín, López-Cuellar, Ma. del Rocío, Flores-Sánchez, Araceli, Vergara-Porras, Berenice
Format Journal Article
LanguageEnglish
Published Cairo, Egypt Hindawi Publishing Corporation 01.01.2017
Hindawi
Hindawi Limited
Subjects
Analytical chemistry
Avocado oil
Bacteria
Biodegradable materials
Biosynthesis
Biotechnology
Carbon
Enzymes
Fatty acids
Fermentation
Fourier transforms
Fructose
Gas chromatography
Mechanical properties
Metabolism
Molecular structure
Polymers
Sugarcane
Synthesis
Thermodynamic properties
Vegetable oils
Mexico
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Summary:Poly-R-hydroxyalkanoates (PHAs) are polymers produced by a vast number of bacterial species under stress conditions. PHAs exhibit different thermal and mechanical properties that depend on their molecular structure. In this work, PHAs were produced using avocado oil as the carbon source. Cupriavidus necator H16 was cultured in three-stage fermentation using fructose during the cell growth stages and avocado oil during the PHA synthesis stage. Different concentrations of avocado oil were used during the third stage to test the incorporation of various monomeric units into the PHAs. Biomass and PHA production were measured during the fermentation. DSC, FTIR, and gas chromatography analysis aided the PHA characterization. Different proportions of 3-hydroxyvalerate were present in the 3-hydroxybutyrate main chain depending on the concentration of avocado oil. The results suggest that avocado oil is a viable new substrate for PHA production.
ISSN:1687-9422
1687-9430
DOI:10.1155/2017/6942950