In vivo evaluation of porous hydroxyapatite/chitosan–alginate composite scaffolds for bone tissue engineering

• Published in: International journal of biological macromolecules Vol. 51; no. 5; pp. 1079 - 1085
• Main Authors: Jin, Hyeong-Ho, Kim, Dong-Hyun, Kim, Tae-Wan, Shin, Keun-Koo, Jung, Jin Sup, Park, Hong-Chae, Yoon, Seog-Young
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2012
• Subjects: Alginate; Alginates - metabolism; Animals; biocompatibility; Biocompatible Materials - chemistry; Biocompatible Materials - metabolism; Biocompatible Materials - pharmacology; Biocompatible Materials - toxicity; Bone and Bones - cytology; Bone and Bones - physiology; bone formation; Cell Line, Tumor; Chitosan; Chitosan - metabolism; coprecipitation; cytotoxicity; Durapatite - metabolism; Freeze Drying; Glucuronic Acid - metabolism; Hexuronic Acids - metabolism; Humans; Hydroxyapatite; In vivo; in vivo studies; Mice; Osteogenesis - drug effects; Porosity; Scaffold; skull; tissue engineering; Tissue Engineering - methods; Tissue Scaffolds; In vivo; Hydroxyapatite; Alginate; Chitosan; Scaffold
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2012.08.027

Porous hydroxyapatite (HAp)/chitosan–alginate composite scaffolds were prepared through in situ co-precipitation and freeze-drying for bone tissue engineering. The composite scaffolds were highly porous and interconnected with a pore size of around 50–220μm at low concentrations of HAp. As the HAp content increased, the porosity of the scaffolds decreased from 84.98 to 74.54%. An MTT assay indicates that the obtained scaffolds have no cytotoxic effects on MG-63 cells, and that they have good biocompatibility. An implantation experiment in mouse skulls revealed that the composite scaffold provides a strong positive effect on bone formation in vivo in mice. Furthermore, that HAp/chitosan–alginate composite scaffold has been shown to be more effective for new bone generation than chitosan–alginate scaffold.