Identification and expression pattern analysis of the glucosinolate biosynthetic gene BoCYP83B1 from broccoli
Glucosinolates are a branch of amino acid-derived metabolites, which are specifically found in Brassicales . In Arabidopsis , tryptophan derived indolic glucosinolates are required for plant defense against a wide range of pathogens and herbivores due to their strong antimicrobial activity and poten...
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Published in | Biologia plantarum Vol. 62; no. 3; pp. 521 - 533 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer Netherlands
01.09.2018
Springer Nature B.V Institute of Experimental Botany of the Czech Academy of Sciences |
Subjects | |
Online Access | Get full text |
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Summary: | Glucosinolates are a branch of amino acid-derived metabolites, which are specifically found in
Brassicales
. In
Arabidopsis
, tryptophan derived indolic glucosinolates are required for plant defense against a wide range of pathogens and herbivores due to their strong antimicrobial activity and potential signaling function. An important enzyme in indolic glucosinolate biosynthesis pathway is
CYP83B1
, which oxidizes indole-3-acetaldoxime, a precursor of indole-3-acetic acid (IAA). In this study, we reported isolation and expression characterization of a
CYP83B1
gene from
Brassica oleracea
L. var.
italica
Plenck, which we termed
BoCYP83B1
. Overexpression of
BoCYP83B1
in
Arabidopsis
resulted in an altered glucosinolate profile and early flowering phenotype. By expressing the reporter gene β-glucuronidase under the control of the
BoCYP83B1
promoter in Arabidopsis, we analyzed the spatial expression pattern of
BoCYP83B1
under normal growth conditions as well as in response to several hormones and stresses. The
BoCYP83B1
was primarily expressed in vascular tissue through the almost whole plant. It was strongly induced by methyl jasmonate, 1-amino-1-cyclopropanecarboxylic acid, salicylic acid (SA), gibberellin, and IAA, suggesting its involvement in complex signaling pathways. Mannitol, NaCl, UV, and Flagelin 22 significantly up-regulated
BoCYP83B1
expression, indicating its possible role in stress response. Interestingly, the response of
BoCYP83B1
to SA and NaCl showed tissue specificity. Thus,
BoCYP83B1
might have different functions in different tissues. |
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ISSN: | 0006-3134 1573-8264 |
DOI: | 10.1007/s10535-018-0797-0 |