Analysis of 13 Fentanils, Including Sufentanil and Carfentanil, in Human Urine by Liquid Chromatography-Atmospheric-Pressure Ionization-Tandem Mass Spectrometry

A sensitive, semiautomated method for the analysis of several fentanils and fentanil metabolites in human urine is described. This method uses solid-phase extraction of urine samples followed by liquid chromatography-atmospheric pressure ionization-tandem mass spectrometry. Isotopically labeled inte...

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Bibliographic Details
Published inJournal of analytical toxicology Vol. 30; no. 5; pp. 335 - 341
Main Authors Wang, Lanqing, Bernert, John T.
Format Journal Article
LanguageEnglish
Published England Oxford University Press 01.06.2006
Subjects
Analgesics, Opioid - urine
Chromatography, High Pressure Liquid
Fentanyl - analogs & derivatives
Fentanyl - urine
Humans
Mass Spectrometry
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Summary:A sensitive, semiautomated method for the analysis of several fentanils and fentanil metabolites in human urine is described. This method uses solid-phase extraction of urine samples followed by liquid chromatography-atmospheric pressure ionization-tandem mass spectrometry. Isotopically labeled internal standards were included for 6 of the 13 analytes in this study. Estimated detection limits ranged from 3 to 27 pg/mL, and good accuracy and long-term precision were observed in most cases. This method should be useful for the rapid and sensitive screening of human urine samples for a number of fentanils and their metabolites as indicators of exposure.
ISSN:0146-4760
1945-2403
DOI:10.1093/jat/30.5.335