Molecular characterization of a putative heat shock protein cognate gene in Rhynchosciara americana

• Published in: Chromosome research Vol. 17; no. 7; pp. 935 - 945
• Main Authors: de Andrade, Alexandre, Siviero, Fabio, Rezende-Teixeira, Paula, Santelli, Roberto Vicente, Machado-Santelli, Glaucia Maria
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.10.2009; Springer Netherlands; Springer Nature B.V
• Subjects: Animal Genetics and Genomics; Animals; Biomedical and Life Sciences; Cell Biology; Cell Nucleus - metabolism; Cytoplasm - metabolism; Diptera; Diptera - genetics; Diptera - metabolism; Gene Expression Profiling; Gene Expression Regulation; Genes; Genomics; Heat-Shock Response; HSC70 Heat-Shock Proteins - genetics; HSC70 Heat-Shock Proteins - metabolism; Human Genetics; Humans; Insect Proteins - genetics; Insect Proteins - metabolism; Insects; Life Sciences; Molecular biology; Phylogeny; Plant Genetics and Genomics; Proteins; Rhynchosciara; Studies; polytene chromosome; development; heat shock protein; DNA puff
• ISSN: 0967-3849; 1573-6849
• DOI: 10.1007/s10577-009-9081-7

An hsc70 homologue gene (Rahsc70) of the diptera Rhynchosciara americana was isolated and characterized. We were able to determine the mRNA sequence from an EST of salivary gland cDNA library, and a Rahsc70 cDNA cassette was used as a probe to isolate the genomic region from a genomic library. The mRNA expression of this gene parallels the 2B puff expansion, suggesting its involvement in protein processing, since this larval period corresponds to a high synthetic activity period. During heat shock stress conditions, hsc70 expression decreased. In situ hybridization of polytene chromosomes showed that the Rahsc70 gene is located near the C3 DNA puff. The cellular localization of Hsc70 protein showed this protein in the cytoplasm and in the nucleus.