Targetability of hyaluronic acid nanogel to cancer cells: In vitro and in vivo studies

We have, in previous work developed, characterized and evaluated the biocompatibility of an engineered hyaluronic acid nanogel. Here we assess the targetability of a hyaluronic acid nanogel towards CD44 overexpressing cells, in vitro and in vivo. Results obtained by flow cytometry and confocal fluor...

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Bibliographic Details
Published inEuropean journal of pharmaceutical sciences Vol. 104; pp. 102 - 113
Main Authors Pedrosa, S.S., Pereira, P., Correia, A., Gama, F.M.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 15.06.2017
Subjects
A549 Cells
Animals
Cancer nanotechnology
Carcinoma, Non-Small-Cell Lung - drug therapy
Carcinoma, Non-Small-Cell Lung - metabolism
Carcinoma, Non-Small-Cell Lung - pathology
Gels
Humans
Hyaluronic acid
Hyaluronic Acid - administration & dosage
Hyaluronic Acid - pharmacokinetics
In Vitro Techniques
Kidney - metabolism
Liver - metabolism
Lung Neoplasms - drug therapy
Lung Neoplasms - metabolism
Lung Neoplasms - pathology
Male
Mice, Nude
Nanotechnology
Non-invasive imaging
Non-small cancer lung cells
Skin - metabolism
Spectroscopy, Near-Infrared
Tissue Distribution
Cancer nanotechnology
Non-small cancer lung cells
Non-invasive imaging
Hyaluronic acid
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Summary:We have, in previous work developed, characterized and evaluated the biocompatibility of an engineered hyaluronic acid nanogel. Here we assess the targetability of a hyaluronic acid nanogel towards CD44 overexpressing cells, in vitro and in vivo. Results obtained by flow cytometry and confocal fluorescence microscopy shows that nanogel is greatly internalized by non-small cancer lung cells (A549 cells), that overexpress CD44 receptors. The biodistribution and tumor targetability of the nanogel labelled with a near-infrared (NIR) probe were performed, in mice, through a non-invasive imaging system. Results revealed nanogel high targetability towards an induced subcutaneous A549 tumor. Nanogels pharmacokinetics was evaluated also in healthy animals, and Alexa Fluor 680 labelled nanogel exhibited higher accumulation in liver, kidneys and skin. Also, a comparative biodistribution study was performed, using two NIR imaging probes, Cy5.5 and Alexa Fluor 680. [Display omitted]
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0928-0987
1879-0720
DOI:10.1016/j.ejps.2017.03.045