Protons and single nucleotide polymorphism detection: A simple use for the Ion Sensitive Field Effect Transistor

The identification of single nucleotide polymorphisms (SNP) is a subject receiving considerable attention owing to the possibility of diagnosing and screening for diseases they are connected with. Currently, the emphasis for their detection is using DNA hybridisation methods and the development of n...

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Published inSensors and actuators. B, Chemical Vol. 114; no. 2; pp. 964 - 968
Main Authors Purushothaman, Sunil, Toumazou, Chris, Ou, Chung-Pei
Format Journal Article
LanguageEnglish
Published Elsevier B.V 26.04.2006
Subjects
DNA
DNA sequencing
Genetic diagnosis
ISFET
Real time sequencing
Testing
ISFET
MOSFET
SNP
DNA
dNTP
pH
Real time sequencing
DNA sequencing
Genetic diagnosis
Testing
Online AccessGet full text
ISSN0925-4005
1873-3077
DOI10.1016/j.snb.2005.06.069

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Summary:The identification of single nucleotide polymorphisms (SNP) is a subject receiving considerable attention owing to the possibility of diagnosing and screening for diseases they are connected with. Currently, the emphasis for their detection is using DNA hybridisation methods and the development of novel, mainly fluorescent based methods for hybridisation detection. We report on a new method for identifying SNPs using Ion Sensitive Field Effect Transistor technology. Through the detection of single nucleotide mismatches, we show a procedure for effectively sequencing one base and therefore a mechanism for sequencing new DNA.
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ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2005.06.069