Methods and Applications of Three-Color Confocal Imaging

A simple method for constructing two and three-color merged images from grayscale confocal fluorescence images using Adobe Photoshop is outlined. Various computer methods for manipulating and displaying the images are discussed in light of several recent biomedical applications of multi-label confoc...

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Bibliographic Details
Published inBioTechniques Vol. 22; no. 1; pp. 120 - 126
Main Authors Paddock, Stephen W, Hazen, Eric J, DeVries, Peter J
Format Journal Article
LanguageEnglish
Published Natick, MA Future Science Ltd 01.01.1997
Eaton
Taylor & Francis Group
Subjects
Animals
Biological and medical sciences
Drosophila - embryology
Embryo, Nonmammalian - cytology
Fluorescence
Image Processing, Computer-Assisted - methods
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Microscopy, Confocal - methods
Miscellaneous. Technology
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Software
Videotape Recording
Fluorescence
Confocal microscopy
Application
Imaging
Medical application
Computer aid
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Summary:A simple method for constructing two and three-color merged images from grayscale confocal fluorescence images using Adobe Photoshop is outlined. Various computer methods for manipulating and displaying the images are discussed in light of several recent biomedical applications of multi-label confocal microscopy.
ISSN:0736-6205
1940-9818
DOI:10.2144/97221st04