Proteomic analysis of kidney in rats chronically exposed to fluoride

• Published in: Chemico-biological interactions Vol. 180; no. 2; pp. 305 - 311
• Main Authors: Kobayashi, Claudia A.N., Leite, Aline L., Silva, Thelma L., Santos, Lucilene D., Nogueira, Fábio C.S., Oliveira, Rodrigo C., Palma, Mario S., Domont, Gilberto B., Buzalaf, Marília A.R.
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 15.07.2009
• Subjects: Animals; Dose-Response Relationship, Drug; Drug Administration Schedule; Electrophoresis, Gel, Two-Dimensional; Fluoride; Fluorides - administration & dosage; Fluorides - toxicity; Gene Expression Profiling; Gene Expression Regulation - drug effects; Kidney; Kidney - drug effects; Kidney - metabolism; Male; Mass spectrometry; Metabolism; Proteomics; Rats; Two-dimensional gel electrophoresis; Fluoride; Metabolism; Mass spectrometry; Kidney; Proteomics; Two-dimensional gel electrophoresis
• ISSN: 0009-2797; 1872-7786
• DOI: 10.1016/j.cbi.2009.03.009

Two-dimensional gel electrophoresis (2-DE) was used to better understand alterations in renal metabolism induced by fluoride (F). Three groups of weanling male Wistar rats were treated with drinking water containing 0 (control), 5, or 50 ppm F for 60 days ( n = 6/group). Kidneys were collected for proteomic and histological (HE) analysis. After protein isolation, renal proteome profiles were examined using 2-DE and Colloidal Coomassie Blue staining. Protein spots with a 2-fold significant difference as detected by quantitative intensity analysis (Image Master Platinum software) and t-test ( p < 0.05) were excised and analyzed by MALDI-TOF MS (matrix assisted laser desorption ionization-time-of-flight mass spectrometry). The histological analysis revealed no damage in kidneys induced by F, except for a vascular congestion in the 50 ppm F group. Between control vs 50 ppm F, and control vs 5 ppm F groups, 12 and 6 differentially expressed proteins were detected, respectively. Six proteins, mainly related with metabolism, detoxification and housekeeping, were successfully identified. At the high F group, pyruvate carboxylase, a protein involved in the formation of oxaloacetate was found to be downregulated, while enoyl coenzyme A hydratase, involved in fatty acids oxidation, was found to be upregulated. Thus, proteomic analysis can provide new insights into the alterations in renal metabolism after F exposure, even in low doses.