Revisiting the phylogeny of Bombacoideae (Malvaceae): Novel relationships, morphologically cohesive clades, and a new tribal classification based on multilocus phylogenetic analyses

• Published in: Molecular phylogenetics and evolution Vol. 101; pp. 56 - 74
• Main Authors: Carvalho-Sobrinho, Jefferson G., Alverson, William S., Alcantara, Suzana, Queiroz, Luciano P., Mota, Aline C., Baum, David A.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.08.2016
• Subjects: Adansonia; Baobab; Bayes Theorem; Bayesian theory; Bombax; Bombax ceiba; calyx; Ceiba; Databases, Genetic; DNA; endocarp; forests; Fruit traits; Genetic Loci; internal transcribed spacers; kapok; Kapok group; Malvaceae; Malvaceae - anatomy & histology; Malvaceae - classification; Malvaceae - genetics; Neotropics; Pachira; Phylogeny; plant spines; Quantitative Trait, Heritable; Scleronema; Seeds - anatomy & histology; synapomorphy; taxonomic keys; Tribal classification; tropics; Baobab; Fruit traits; Kapok group; Neotropics; Tribal classification
• ISSN: 1055-7903; 1095-9513; 1095-9513
• DOI: 10.1016/j.ympev.2016.05.006

[Display omitted] •We reconstructed a phylogeny of Bombacoideae (Malvaceae) based on plastid and nuclear ribosomal DNA.•Novel phylogenetic relationships emerged including clades that are morphologically cohesive based on fruit and seed traits.•The phylogenetic placement of Pochota fendleri, a historically incertae sedis taxon, is resolved.•We present a new tribal classification and an identification key to the tribes. Bombacoideae (Malvaceae) is a clade of deciduous trees with a marked dominance in many forests, especially in the Neotropics. The historical lack of a well-resolved phylogenetic framework for Bombacoideae hinders studies in this ecologically important group. We reexamined phylogenetic relationships in this clade based on a matrix of 6465 nuclear (ETS, ITS) and plastid (matK, trnL-trnF, trnS-trnG) DNA characters. We used maximum parsimony, maximum likelihood, and Bayesian inference to infer relationships among 108 species (∼70% of the total number of known species). We analyzed the evolution of selected morphological traits: trunk or branch prickles, calyx shape, endocarp type, seed shape, and seed number per fruit, using ML reconstructions of their ancestral states to identify possible synapomorphies for major clades. Novel phylogenetic relationships emerged from our analyses, including three major lineages marked by fruit or seed traits: the winged-seed clade (Bernoullia, Gyranthera, and Huberodendron), the spongy endocarp clade (Adansonia, Aguiaria, Catostemma, Cavanillesia, and Scleronema), and the Kapok clade (Bombax, Ceiba, Eriotheca, Neobuchia, Pachira, Pseudobombax, Rhodognaphalon, and Spirotheca). The Kapok clade, the most diverse lineage of the subfamily, includes sister relationships (i) between Pseudobombax and “Pochota fendleri” a historically incertae sedis taxon, and (ii) between the Paleotropical genera Bombax and Rhodognaphalon, implying just two bombacoid dispersals to the Old World, the other one involving Adansonia. This new phylogenetic framework offers new insights and a promising avenue for further evolutionary studies. In view of this information, we present a new tribal classification of the subfamily, accompanied by an identification key.