Characterization of Thermophilic Microorganisms in the Geothermal Water Flow of El Chichón Volcano Crater Lake

This study reports for the first time the isolation, identification and characterization of lipase-producing thermophilic strain from the geothermal water of the El Chichón volcano crater lake. Two strains were identified by 16S rRNA sequencing as Geobacillus jurassicus CHI2 and Geobacillus stearoth...

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Bibliographic Details
Published inWater (Basel) Vol. 12; no. 8; p. 2172
Main Authors Ovando-Chacon, Sandy Luz, Tacias-Pascacio, Veymar Guadalupe, Ovando-Chacon, Guillermo Efren, Rosales-Quintero, Arnulfo, Rodriguez-Leon, Abelardo, Ruiz-Valdiviezo, Victor Manuel, Servin-Martinez, Alberto
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.08.2020
Subjects
Analysis
Bacteria
Bacteria, Thermophilic
Biocatalysts
Casein
Craters
Enzymes
Flagella
Fructose
Genetic aspects
geothermal flow
Geothermal resources
Heat transfer
High temperature
hydrolysis
Identification and classification
Lakes
Lipase
Maltose
Microorganisms
Olive oil
RNA sequencing
rRNA 16S
Starch
Strains (organisms)
Sucrose
Sugar
temperature gradient
thermal biotechnological processes
Thermophilic bacteria
Thermophilic microorganisms
Volcanoes
Water flow
Mexico
United States > US
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Summary:This study reports for the first time the isolation, identification and characterization of lipase-producing thermophilic strain from the geothermal water of the El Chichón volcano crater lake. Two strains were identified by 16S rRNA sequencing as Geobacillus jurassicus CHI2 and Geobacillus stearothermophilus CHI1. Results showed that G. jurassicus CHI2 is Gram-positive, able to ferment maltose, fructose and sucrose and to hydrolyze starch and casein; while G. stearothermophilus CHI1 showed to be Gram-variable, able to ferment maltose and fructose and to hydrolyze starch. Colonies of both strains presented irregular shape, umbilicated elevation of gummy texture and cells presented flagellar movement to survive in fluids with high temperature and mass gradients due to complex phenomena of heat and mass transfer present in the geothermal fluids. Lipase production for G. stearothermophilus CHI1 was also evaluated. It was found that this strain possesses a growth associated with extracellular lipase production with a high activity of 143 U/mL at 8.3 h of incubation time, superior to the activities reported for other microorganisms of genus Geobacillus; for this reason, it can be said that the thermal flow of the El Chichón volcano crater lake can be a useful source of lipase-producing thermophilic bacteria.
ISSN:2073-4441
2073-4441
DOI:10.3390/w12082172