Long Chain Perfluorinated Alkyl Acids Derivatisation and Identification in Biota and Abiota Matrices Using Gas Chromatography

• Published in: Bulletin of environmental contamination and toxicology Vol. 83; no. 5; pp. 630 - 635
• Main Authors: Orata, Francis, Quinete, Natalia, Wilken, Rolf-Dieter
• Format: Journal Article
• Language: English
• Published: New York New York : Springer-Verlag 01.11.2009; Springer-Verlag; Springer Nature B.V
• Subjects: Acids - chemistry; Animals; Aquatic Pollution; Biota; Calibration; Chemical compounds; Chromatography; Chromatography, Gas - methods; Contamination; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Esterification; Fishes; Fluorocarbons - analysis; Fluorocarbons - chemistry; Gas chromatography; Gas Chromatography-Mass Spectrometry; Mass spectrometry; Mass spectroscopy; Pollution; Soil Science & Conservation; Waste Water Technology; Water Management; Water Pollutants, Chemical - analysis; Water Pollution Control; Water; Fish muscles; Benzyl esters; Perfluorinated alkyl acids; GC/MS; Derivatisation
• ISSN: 0007-4861; 1432-0800
• DOI: 10.1007/s00128-009-9756-y

An analytical method involving derivatisation of perfluorocarboxylic acids for their analysis in abiotic and biotic matrices is presented. Derivatisation of the acid group to form a suitable alkyl ester provided a suitable compound for mass spectrometric detection in gas chromatography/mass spectroscopy (GC/MS) instrumental analysis. The acid is esterified by an alkyl halide i.e. benzyl bromide as the alkylating agent for perfluorocarboxylic acids quantification in fish and water by GC/MS. The gas chromatography method can be applied in the analysis perfluoro alkyl acids in water and biological matrices, especially where high levels of these compounds are expected. Typical values for precision obtained were 0.1%-10.0% with concentrations ranging from 0.2 to 1 μg/mL). Results demonstrate that GC/MS can supplement liquid chromatographic/mass spectroscopy method for quantification of fluorocarboxylic acid surfactants. The result indicates that there is need for more research on method analysis of perfluorinated acids in environmental matrices.