Structure and Polymorphism of the Mouse Myelin/Oligodendrocyte Glycoprotein Gene

• Published in: Genomics (San Diego, Calif.) Vol. 23; no. 1; pp. 36 - 41
• Main Authors: Daubas, Philippe, Pham-Dinh, Danielle, Dautigny, André
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.09.1994; Elsevier
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; Exons; Fundamental and applied biological sciences. Psychology; Genes; Genes. Genome; Membrane Glycoproteins - genetics; Mice - genetics; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Myelin Proteins - genetics; Myelin-Associated Glycoprotein; Myelin-Oligodendrocyte Glycoprotein; Polymorphism, Genetic; Promoter Regions, Genetic; Genetic marker; Nucleotide sequence; Myelin; Transcription promoter; Rodentia; Central nervous system; Gene organization; Glycoproteins; Lipoprotein; Vertebrata; Mammalia; Oligodendrocyte; Mouse; Microsatellite DNA; Chromosome DNA; Brain (vertebrata); Polymorphism
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1006/geno.1994.1456

We have isolated and characterized genomic clones containing the mouse myelin/oligodendrocyte glycoprotein (MOG) gene. It spans a region of 12.5 kb and consists of eight exons. Its exon-intron structure differs from that of classical MHC-class I genes, with which it is linked in the mouse genome. Nucleotide sequencing of the 5' flanking region reveals that it contains several putative protein-binding sites, some of them in common with other myelin gene promoters. One intragenic polymorphism has been identified: it consists of a GA repeat, defining at least three alleles in mouse inbred strains, and is easily detectable using the polymerase chain reaction method.