Oceanicoccus sagamiensis gen. nov., sp. nov., a Gammaproteobacterium Isolated from Sea Water of Sagami Bay in Japan

• Published in: The journal of microbiology Vol. 49; no. 2; pp. 233 - 237
• Main Authors: Park, S.H., The University of Tokyo, Tokyo, Japan, Yoshizawa, Susumu, The University of Tokyo, Chiba, Japan, Kogure, Kazuhiro, The University of Tokyo, Chiba, Japan, Yokota, Akira, The University of Tokyo, Tokyo, Japan
• Format: Journal Article
• Language: English
• Published: Heidelberg The Microbiological Society of Korea 01.04.2011; Springer Nature B.V; 한국미생물학회
• Subjects: AMBIENTE MARINO; Base Composition; Biomedical and Life Sciences; Cloning; Cluster Analysis; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Fatty Acids - analysis; Gammaproteobacteria; Gammaproteobacteria - classification; Gammaproteobacteria - genetics; Gammaproteobacteria - isolation & purification; Gammaproteobacteria - physiology; Japan; Life Sciences; Locomotion; MARINE ENVIRONMENT; Microbiology; Microscopy; MILIEU MARIN; Molecular Sequence Data; Oceanicoccus sagamiensis gen. nov., sp. nov; Phylogeny; Quinones - analysis; RNA, Ribosomal, 16S - genetics; Seawater; Seawater - microbiology; Sequence Analysis, DNA; 생물학; Japan; marine environment; gen. nov; sp. nov
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-011-0368-y

A gram-negative, motile, coccoid- and amorphous-shaped, non-pigmented chemoheterotrophic bacterium, designated strain PZ-5∨T, was isolated from sea water of Sagami Bay in Japan and subjected to a polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the novel isolate could be affiliated with the class Gammaproteobacteria. Strain PZ-5∨T showed below 93.9% similarity with validly published bacteria and demonstrated the highest sequence similarity to Dasania marina KOPRI 20902∨T (93.9%). Strain PZ-5∨T formed a monophyletic group with D. marina KOPRI 20902∨T. The DNA G+C content of strain PZ-5∨T was 49.8 mol%. The major isoprenoid quinone was Q-8 and predominant cellular fatty acids were C∧15:0 ISO 2OH (19%), C∧16:1 ω7c (17.4%), C∧17:1 ω8c (16.2%), C∧11:0 3OH (7.5%), and C∧15:1 ω8c (6.5%). Based on evidence from a polyphasic taxonomical study, it was concluded that the strain should be classified as representing a new genus and species of the class Gammaproteobacteria, for which the name Oceanicoccus sagamiensis gen. nov., sp. nov., (type strain PZ-5∨T =NBRC 107125∨T =KCTC 23278∨T) is proposed.