Clonal variation within a mucosal isolate derived from a patient with Leishmania (viannia) braziliensis infection

• Published in: Revista do Instituto de Medicina Tropical de São Paulo Vol. 33; no. 5; pp. 343 - 350
• Main Authors: CUBA-CUBA, C. A, EVANS, D, ROSA, A. DE C, MARSDEN, P. D
• Format: Journal Article
• Language: English
• Published: São Paulo Instituto de Medicina Tropical de São Paulo 01.09.1991; Instituto de Medicina Tropical
• Subjects: Animals; Antibodies, Monoclonal; Antigenic Variation; Biological and medical sciences; Biomarkers - analysis; Cricetinae; Electrophoresis, Cellulose Acetate; Fluorescent Antibody Technique; Human protozoal diseases; Humans; Infectious diseases; Isoenzymes - analysis; Leishmania braziliensis - enzymology; Leishmaniasis, Mucocutaneous - genetics; Leishmaniasis, Mucocutaneous - parasitology; Leshmaniasis; Medical sciences; Parasitic diseases; Protozoal diseases; TROPICAL MEDICINE; South America; Brazil; America; Infection; Protozoal disease; Vertebrata; Experimental disease; Mammalia; Animal; Rodentia; Phenotype variation; Mucocutaneous; Leishmaniasis; Hamster; Clone; Leishmania (Viannia) braziliensis; Clonal Variation; Serodemes; Zymodemes
• ISSN: 0036-4665; 1678-9946; 1678-9946; 0036-4665
• DOI: 10.1590/S0036-46651991000500002

Three isolates over 5 years from a patient with persistent relapsing mucosal leishmaniasis due to Leishmania (Viannia) braziliensis and 7 clones from one of these isolates were studied by zymodemes and serodemes analysis. Results showed evidences of clonal phenotypic variation. Eight isoenzymes markers demonstrated clear differences on Cellulose Acetate (CA) and thin starch gel electrophoresis. Also a panel of specific monoclonal antibodies showed such differences. Our observations provide additional evidence that Leishmania (Viannia) braziliensis is composed by subpopulations of parasites with peculiar biochemical and antigenic characteristics.