Simultaneous determination of 17 antibacterials in chicken tissues by high performance liquid chromatography

• Published in: Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) Vol. 29; no. 1; pp. 13 - 20_1
• Main Authors: Ngata, T. (Ibaraki-ken. Hygienic Lab., Mito (Japan)), Saeki, M
• Format: Journal Article
• Language: Japanese; English
• Published: Japanese Society for Food Hygiene and Safety 1988
• Subjects: ANALISIS QUIMICO; ANALYSE CHIMIQUE; CHEMICAL ANALYSIS; chicken tissue; CHICKENS; CHROMATOGRAPHIE LIQUIDE HAUTE PRESS; clopidol; CROMATOGRAFIA LIQUIDA ALTA PRESION; decoquinate; DRUGS; ethopabate; furan derivatives; high performance liquid chromatography; HIGH PRESSURE LIQUID CHROMATOGRAPHY; MEDICAMENT; MEDICAMENTOS; nicarbazin; POLLO; POULET; sulfa drugs; synthetic antibacterials; trimethoprim
• ISSN: 0015-6426; 1882-1006
• DOI: 10.3358/shokueishi.29.13

A high performance liquid chromatographic method (HPLC) for determination of 7 sulfonamides, clopidol, trimethoprim, ormethoprim, furazolidone, dinitolmide, pyrimethamine, ethopabate, difurazone, nicarbazin, and decoquinate, in chicken tissues was developed. The drugs were extracted from tissues by homogenizing with warm methanol. The homogenate was centrifuged and the supernatant was evaporated to dryness. The residue was dissolved in buffer solution (pH 5.5) and partitioned with n-hexane. The buffer solution was then partitioned with chloroform. The chloroform layer was evaporated to dryness and the residue was dissolved in 85% acetonitrile and applied to an alumina column. The drugs were eluted with 85% acetonitrile and the eluate was applied to an HPLC column packed with Nucleosil 5C18. Decoquinate was eluted with methanol-water (9:1; detected at 315nm), difurazone and nicarbazine with acetonitrile-acetic acid-water (50:0.5:50; detected at 350nm), and the other drugs with acetonitrile-acetic acid-water (14:0.5:86; detected at 270nm and at 350nm). The average recoveries of all drugs added at the level of 0.2ppm were more than 65.5%. The detection limits of clopidol, difurazone, nicarbazin, sulfamerazine and sulfamethoxy-pyridazine were 0.03ppm, and those of the other drugs were 0.05ppm.