Defective processing of ribosomal precursor RNA in Saccharomyces cerevisiae

• Published in: Biochemical journal Vol. 220; no. 2; pp. 461 - 467
• Main Authors: Mitlin, J.A, Cannon, M
• Format: Journal Article
• Language: English
• Published: England 01.06.1984
• Subjects: Centrifugation, Density Gradient; Cytoplasm - metabolism; Electrophoresis, Polyacrylamide Gel; Nucleic Acid Precursors - genetics; Nucleic Acid Precursors - metabolism; RNA Precursors; RNA Processing, Post-Transcriptional; RNA, Fungal - genetics; RNA, Fungal - metabolism; RNA, Ribosomal - genetics; RNA, Ribosomal - metabolism; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Spheroplasts - metabolism; yeasts
• ISSN: 0264-6021; 1470-8728
• DOI: 10.1042/bj2200461

Saccharomyces cerevisiae (strain A224A) has an abnormal distribution of cytoplasmic ribosomal subunits when grown at 36 degrees C, with sucrose-gradient analysis of extracts revealing an apparent excess of material sedimenting at 60 S. This abnormality is not observed at either 23 degrees C or 30 degrees C. At 36 degrees C the defect(s) is expressed as a slowed conversion of 20 S ribosomal precursor RNA to mature 18 S rRNA, although the corresponding maturation of 27 S ribosomal precursor RNA to mature 25 S rRNA is normal. Studies on this yeast strain and on mutants derived from it may help to elucidate the role(s) of individual ribosomal components in controlling ribosome biogenesis in eukaryotes.