Sample preparation and orthogonal chromatography for broad polarity range plasma metabolomics: Application to human subjects with neurodegenerative dementia

• Published in: Analytical biochemistry Vol. 455; pp. 48 - 54
• Main Authors: Armirotti, Andrea, Basit, Abdul, Realini, Natalia, Caltagirone, Carlo, Bossù, Paola, Spalletta, Gianfranco, Piomelli, Daniele
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.06.2014
• Subjects: Aged; Alzheimer disease; Alzheimer Disease - blood; Animals; Biochemistry - methods; Blood Proteins - analysis; Case-Control Studies; chromatography; Chromatography, Liquid - methods; cognitive disorders; Dementia - blood; Dried plasma spot; Female; Humans; hydrophilicity; hydrophobicity; Male; Mass spectrometry; metabolome; Metabolomics; Metabolomics - methods; Mice; Multivariate Analysis; Orthogonal chromatography; Sample preparation; Sphingomyelins - blood; Tandem Mass Spectrometry; Metabolomics; Dried plasma spot; Sample preparation; Mass spectrometry; Orthogonal chromatography

We describe a simple protocol for the preparation and orthogonal hydrophobic/hydrophilic LC–MS/MS analysis of mouse and human plasma samples, which enables the untargeted (“shotgun”) or targeted profiling of hydrophilic, amphipathic, and hydrophobic constituents of plasma metabolome. The protocol is...